Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse

Development
Takayuki TatsumiSatoshi Tsukamoto

Abstract

Although autophagy is classically viewed as a non-selective degradation system, recent studies have revealed that various forms of selective autophagy also play crucial physiological roles. However, the induction of selective autophagy is not well understood. In this study, we established a forced selective autophagy system using a fusion of an autophagy adaptor and a substrate-binding protein. In both mammalian cells and fertilized mouse embryos, efficient forced lipophagy was induced by expression of a fusion of p62 (Sqstm1) and a lipid droplet (LD)-binding domain. In mouse embryos, induction of forced lipophagy caused a reduction in LD size and number, and decreased the triglyceride level throughout embryonic development, resulting in developmental retardation. Furthermore, lipophagy-induced embryos could eliminate excess LDs and were tolerant of lipotoxicity. Thus, by inducing forced lipophagy, expression of the p62 fusion protein generated LD-depleted cells, revealing an unexpected role of LD during preimplantation development.

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Citations

Apr 27, 2018·The Journal of Reproduction and Development·Satoshi Tsukamoto, Takayuki Tatsumi
Dec 28, 2018·Antioxidants & Redox Signaling·Se-Jin JeongBabak Razani
Feb 27, 2020·The Journal of Biological Chemistry·Isao TamuraNorihiro Sugino
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Jul 7, 2020·American Journal of Respiratory and Critical Care Medicine·Maéva ZysmanLaurent Boyer
Oct 20, 2020·The Journal of Reproduction and Development·Ryutaro AizawaSatoshi Tsukamoto
Feb 25, 2021·Biopreservation and Biobanking·Du XingzhuFu Xiangwei
Feb 27, 2021·Scientific Reports·Koji MaekuraMasaoki Takano
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Ranjha KhanQinghua Shi
Feb 11, 2021·The Journal of Reproduction and Development·Asuka Furuta, Toshinobu Nakamura
Jul 3, 2021·International Journal of Molecular Sciences·Marcelo T MouraFabíola F Paula-Lopes

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