Foreign gene expression in the mouse testis by localized in vivo gene transfer

Biochemical and Biophysical Research Communications
T MuramatsuJ Okumura

Abstract

In order to attain foreign gene expression in vivo in the testis of living mice, chloramphenicol acetyltransferase (CAT), firefly luciferase and bacterial lacZ reporter genes were transfected by microparticle bombardment and electroporation. The results showed that CAT reporter gene was expressed in a dose-dependent fashion. The X-gal staining showed that in some spermatogenic-like cells, the bacterial lacZ gene was also expressed by in vivo electroporation, but not by in vivo microparticle bombardment. The possibility of in vivo gene transfer to the spermatogenic cells of the mouse testis was further confirmed by the fact that the CAT reporter gene expression was testis-specific when driven by the mouse-protamin 1 promoter. It was concluded, therefore, that in vivo microparticle bombardment and, especially, electroporation provide convenient and efficient means of gene transfer to the testis of living mice.

References

Mar 1, 1992·Bio/technology·T M KleinS Fitzpatrick-McElligott
Mar 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·H SuemoriN Nakatsuji
Oct 20, 1989·Cell·R L BrinsterR D Palmiter

Citations

Oct 18, 2011·Asian Journal of Andrology·Zhen ZhangFei Zhou
Apr 21, 2001·DNA and Cell Biology·M Lee LucasR Heller
Mar 10, 2001·DNA and Cell Biology·R HellerC Nicolau
Aug 28, 2003·Journal of Virology·Hideki TaniYoshiharu Matsuura
Jul 16, 2008·Reproductive Biology and Endocrinology : RB&E·Emilio González-GonzálezJesús Del Mazo
Oct 20, 2005·Journal of Bioscience and Bioengineering·S RyokiT Muramatsu
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kayoko KuritaNoriyoshi Sakai
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M NaganoR L Brinster
Feb 22, 2012·Expert Opinion on Biological Therapy·Saulius SatkauskasMindaugas S Venslauskas
May 8, 2001·Archives of Andrology·M FujisawaS Kamidono
Sep 14, 2007·Archives of Andrology·Kevin CowardJohn Parrington
Jan 13, 2011·Systems Biology in Reproductive Medicine·John ParringtonJoaquin Gadea
Feb 14, 2002·Kidney International·E Imai, Y Isaka
Jan 18, 2006·Mechanisms of Development·Hajime OginoRobert M Grainger
Jan 5, 2000·The Journal of Experimental Zoology·Y YamazakiK Imoto
Jan 1, 2010·Cancers·Stephen J BeebeRichard Heller
Mar 21, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Terje SvingenPeter Koopman
Jun 16, 2000·FEBS Letters·M NaganoR L Brinster
May 10, 2007·Nature Protocols·Hajime OginoRobert M Grainger
May 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Catalina Ana Rosselló, Miguel Torres
Jun 11, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Dec 20, 2002·Connective Tissue Research·Irene AngeliPaul T Sharpe
Feb 27, 2003·Biology of Reproduction·Maria P De Miguel, Peter J Donovan
Feb 17, 2005·Journal of Andrology·Yukihiro UmemotoKenjiro Kohri
May 17, 2008·Development, Growth & Differentiation·Tomomi Shimogori, Masaharu Ogawa

Related Concepts

Metazoa
Order Coleoptera
Lactrase
Lac Gene
Luciferases
Mouse, Inbred ICRC
Testis
Chloramphenicol O-Acetyltransferase
Gene Expression
Gene Transfer Techniques

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