PMID: 9188830May 9, 1997Paper

Forensic analysis of eleven cyclic antidepressants in human biological samples using a new reversed-phase chromatographic column of 2 microm porous microspherical silica gel

Journal of Chromatography. B, Biomedical Sciences and Applications
E TanakaC Wakasugi

Abstract

A high-performance liquid chromatographic method has been developed for the forensic analysis of eleven frequently used cyclic antidepressant drugs (ADSs) (amitriptyline, amoxapine, clomipramine, desipramine, dosulepine, doxepin, imipramine, maprotiline, melitracen, mianserine and nortriptyline) using a recently developed reversed-phase column with 2 microm particles for the analysis of biological samples. The separation was carried out using two different C8 reversed-phase columns (column 1: 100 mm X 4.6 mm I.D., particle size 2 microm, TSK gel Super-Octyl; column 2: 100 mm X 4.6 mm I.D., particle size 5 microm, Hypersil MOS-C8) for comparison. The mobile phase was composed of methanol-20 mM KH2PO4 (pH 7) (60:40, v/v) and the flow-rate was 0.6 ml/min for both columns. The absorbance of the eluent was monitored at 254 nm. When the eleven drugs were determined, the sensitivity with the 2 microm particles was about five times greater than with the 5 microm particles. Retention times on column 1 were shorter than those on column 2. These results show that the new ODS column packing with a particle size of 2 microm gives higher sensitivity and a shorter analysis time than the conventional ODS column packing when applied to the anal...Continue Reading

References

Nov 1, 1982·Journal of Analytical Toxicology·R L TaylorY H Caplan
Jan 1, 1996·Journal of Analytical Toxicology·A Agarwal, N Lemos

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Citations

Jan 31, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J Trocewicz
Feb 20, 2004·Journal of Colloid and Interface Science·W GuoY J Wang
Jul 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Christine FrahnertKatja Grasmäder
Jun 15, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yasuhide SasajimaYuji Takekoshi
Dec 24, 2010·Bioanalysis·Mohammad N UddinIoannis N Papadoyannis
Jan 31, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yoshihiro SaitoKiyokatsu Jinno
Jul 25, 2006·Forensic Science International : Synergy·Tatsuo ShinozukaEinosuke Tanaka
Sep 21, 2007·Journal of Pharmaceutical and Biomedical Analysis·Jinjing CheYuanguo Cheng
Jul 28, 2005·Journal of Pharmacological and Toxicological Methods·Devasish BoseMayte Gil-Agustí
May 24, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Aleksandra ChmielewskaHenryk Lamparczyk
Jun 16, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Aiying Song
Aug 27, 2004·Critical Reviews in Biotechnology·Pratima Bajpai
Nov 9, 2019·Journal of Forensic Sciences·Sara GarciaMelissa Connor

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