PMID: 9537362Apr 16, 1998Paper

Forespore expression and processing of the SigE transcription factor in wild-type and mutant Bacillus subtilis

Journal of Bacteriology
J JuW G Haldenwang

Abstract

SigmaE is a mother cell-specific transcription factor of sporulating Bacillus subtilis that is derived from an inactive precursor protein (pro-sigmaE). To examine the process that prevents sigmaE activity from developing in the forespore, we fused the sigmaE structural gene (sigE) to forespore-specific promoters (PdacF and PspoIIIG), placed these fusions at sites on the B. subtilis chromosome which translocate into the forespore either early or late, and used Western blot analysis to monitor SigE accumulation and pro-sigmaE processing. sigE alleles, placed at sites which entered the forespore early, were found to generate more protein product than the same fusion placed at a late entering site. SigE accumulation and processing in the forespore were enhanced by null mutations in spoIIIE, a gene whose product is essential for translocation of the distal portion of the B. subtilis chromosome into the forespore. In other experiments, a chimera of pro-sigmaE and green fluorescence protein, previously shown to be unprocessed if it is synthesized within the forespore, was found to be processed in this compartment if coexpressed with the gene for the pro-sigmaE-processing enzyme, SpoIIGA. The need for spoIIGA coexpression is obviated i...Continue Reading

References

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Citations

Jan 5, 2006·Applied and Environmental Microbiology·Yoann Le BretonW G Haldenwang
Jan 24, 2012·Applied and Environmental Microbiology·Wasin Buasri, Watanalai Panbangred
Jul 25, 2013·Environmental Microbiology·Katarína MuchováImrich Barák
Apr 14, 1999·Molecular Microbiology·L KroosY T Yu
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·David W Hilbert, Patrick J Piggot
Dec 15, 2010·The Journal of Biological Chemistry·Patrik FlorekAnthony J Wilkinson
Dec 21, 2013·FEMS Microbiology Reviews·Anna KonovalovaLee Kroos
Apr 2, 2008·The Journal of Biological Chemistry·Daisuke ImamuraLee Kroos
Jul 8, 2020·International Journal of Molecular Sciences·Katarína MuchováImrich Barák
Mar 27, 1999·Biochemical and Biophysical Research Communications·J M ScottW G Haldenwang
Dec 21, 2000·Current Opinion in Microbiology·L Kroos, Y T Yu

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