Forespore-specific transcription of the lonB gene during sporulation in Bacillus subtilis

Journal of Bacteriology
M SerranoU Völker

Abstract

The Bacillus subtilis genome encodes two members of the Lon family of prokaryotic ATP-dependent proteases. One, LonA, is produced in response to temperature, osmotic, and oxidative stress and has also been implicated in preventing sigma(G) activity under nonsporulation conditions. The second is encoded by the lonB gene, which resides immediately upstream from lonA. Here we report that transcription of lonB occurs during sporulation under sigma(F) control and thus is restricted to the prespore compartment of sporulating cells. First, expression of a lonB-lacZ transcriptional fusion was abolished in strains unable to produce sigma(F) but remained unaffected upon disruption of the genes encoding the early and late mother cell regulators sigma(E) and sigma(K) or the late forespore regulator sigma(G). Second, the fluorescence of strains harboring a lonB-gfp fusion was confined to the prespore compartment and depended on sigma(F) production. Last, primer extension analysis of the lonB transcript revealed -10 and -35 sequences resembling the consensus sequence recognized by sigma(F)-containing RNA polymerase. We further show that the lonB message accumulated as a single monocistronic transcript during sporulation, synthesis of which r...Continue Reading

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Citations

Jul 18, 2009·Nature Reviews. Microbiology·Janine KirsteinKürşad Turgay
Nov 25, 2004·European Journal of Biochemistry·Henrike Besche, Peter Zwickl
Aug 12, 2008·Journal of Bacteriology·Lyle A SimmonsGraham C Walker
Oct 26, 2002·Journal of Bacteriology·Carsten L BeckeringMohamed A Marahiel
Jul 18, 2003·Journal of Bacteriology·Christopher A TomasEleftherios T Papoutsakis
Feb 20, 2004·Journal of Bacteriology·Teresa CostaAdriano O Henriques
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·David W Hilbert, Patrick J Piggot
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Susan Gottesman
Jul 21, 2006·Research in Microbiology·Virginie TsilibarisLaurence Van Melderen
Jun 12, 2012·Journal of Structural Biology·L'uboš AmbroEva Kutejová
Jun 3, 2016·Plasmid·Anna KarlowiczIgor Konieczny
Jul 28, 2017·Frontiers in Molecular Biosciences·Alexander K W ElsholzKürşad Turgay
Jun 4, 2004·Journal of Bacteriology·Mónica SerranoAdriano O Henriques
Aug 20, 2021·FEMS Microbiology Reviews·Colin R Harwood, Yoshimi Kikuchi

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