A Whole-Brain Map and Assay Parameter Analysis of Mouse VTA Dopaminergic Activation

BioRxiv : the Preprint Server for Biology
Horea-Ioan IoanasM. Rudin


Ascending dopaminergic projections from neurons located in the Ventral Tegmental Area (VTA) are key to the etiology, dysfunction, and control of motivation, learning, and addiction. Due to evolutionary conservation of this nucleus and the extensive use of mice as disease models, establishing an assay for VTA dopaminergic signalling in the mouse brain is crucial for the translational investigation of neuronal function phenotypes of diseases and interventions. In this article we use optogenetic stimulation for VTA dopaminergic neuron stimulation, in combination with functional Magnetic Resonance Imaging (fMRI), a method widely used in human deep brain imaging. We present the first whole-brain opto-fMRI map of dopaminergic activation in the mouse, and show that VTA dopaminergic system function is consistent with but diverges in a few key aspects from its structure. While the activation map predominantly includes and excludes target areas according to their relative projection densities (e.g. strong activation of the nucleus accumbens and low activation of the hippocampus), it also includes areas for which a structural connection is not well established (such as the dorsomedial striatum). We further detail assay variability with re...Continue Reading

Related Concepts

Computer Software
Biological Markers
Genome-Wide Association Study
Biochemical Pathway
Meta-Analysis (Publications)
Meta Analysis (Statistical Procedure)
Gene Delivery Systems

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Jonas A HospAndreas R Luft
Alcoholism, Clinical and Experimental Research
M S BrodieS B Appel
© 2020 Meta ULC. All rights reserved