PMID: 8943009Nov 26, 1996Paper

Fork-like DNA templates support bypass replication of lesions that block DNA synthesis on single-stranded templates

Proceedings of the National Academy of Sciences of the United States of America
Jean-Sebastien HoffmannG Villani

Abstract

DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly with fork opening, whereas synthesis of the lagging strand essentially takes place on a single-stranded template. The effect of this duality on DNA damage processing by the cellular replication machinery was tested using eukaryotic cell extracts and model DNA substrates containing site-specific DNA adducts formed by the anticancer drug cisplatin or by the carcinogen N-2-acetylaminofluorene. Bypass of both lesions was observed only with fork-like substrates, whereas complete inhibition of DNA synthesis occurred on damaged single-stranded DNA substrates. These results suggest a role for additional accessory factors that permit DNA polymerases to bypass lesions when present in fork-like DNA.

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D BurnoufR P Fuchs
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·J S HoffmannG Villani
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·D C ThomasR P Fuchs
Jan 1, 1993·Molecular and Cellular Biology·M P CartyK Dixon
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·D L Svoboda, J M Vos
Jun 28, 1996·The Journal of Biological Chemistry·L A Verkruyse, S L Hofmann

❮ Previous
Next ❯

Citations

Jun 19, 2001·Mutation Research·M Kartalou, J M Essigmann
Jan 8, 2000·Journal of Inorganic Biochemistry·X LinS B Howell
Jan 8, 2000·Journal of Inorganic Biochemistry·S G Chaney, A Vaisman
Nov 1, 1996·Chemistry & Biology·P Karran, M Bignami
May 7, 2010·Journal of the American Chemical Society·Wee Han AngStephen J Lippard
Dec 3, 2013·Virchows Archiv : an International Journal of Pathology·Shigeyuki AsanoMasaru Kojima
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y CanitrotJ S Hoffmann
Oct 12, 2010·Seminars in Cancer Biology·Jean-Sébastien Hoffmann, Christophe Cazaux
Sep 7, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Y CanitrotC Cazaux
Aug 9, 2002·Molecular and Cellular Biology·Maria T A OcampoGeorge W Teebor
Oct 22, 2002·The Journal of Biological Chemistry·Laurence ServantJean-Sébastien Hoffmann
May 23, 1997·The Journal of Biological Chemistry·M Cordeiro-StoneW K Kaufmann
Feb 18, 1999·Molecular and Cellular Biology·A M CordonnierR P Fuchs
Aug 16, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·W. David SedwickMartina L. Veigl

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.