Forkhead Transcription Factor FOXO1 Inhibits Angiogenesis in Gastric Cancer in Relation to SIRT1

Cancer Research and Treatment : Official Journal of Korean Cancer Association
Sue Youn KimByung Lan Lee

Abstract

We previously reported that forkhead transcription factors of the O class 1 (FOXO1) expression in gastric cancer (GC) was associated with angiogenesis-related molecules. However, there is little experimental evidence for the direct role of FOXO1 in GC. In the present study, we investigated the effect of FOXO1 on the tumorigenesis and angiogenesis in GC and its relationship with SIRT1. Stable GC cell lines (SNU-638 and SNU-601) infected with a lentivirus containing FOXO1 shRNA were established for animal studies as well as cell culture experiments. We used xenograft tumors in nude mice to evaluate the effect of FOXO1 silencing on tumor growth and angiogenesis. In addition, we examined the association between FOXO1 and SIRT1 by immunohistochemical tissue array analysis of 471 human GC specimens and Western blot analysis of xenografted tumor tissues. In cell culture, FOXO1 silencing enhanced hypoxia inducible factor-1α (HIF-1α) expression and GC cell growth under hypoxic conditions, but not under normoxic conditions. The xenograft study showed that FOXO1 downregulation enhanced tumor growth, microvessel areas, HIF-1α activation and vascular endothelial growth factor (VEGF) expression. In addition, inactivated FOXO1 expression was ...Continue Reading

References

Jun 8, 1999·The Journal of Biological Chemistry·E D TangK L Guan
Apr 15, 2003·The Journal of Pathology·Hye Seung LeeWoo Ho Kim
Jun 9, 2004·The Journal of Biological Chemistry·Tatsuo FuruyamaNozomu Mori
Jun 17, 2004·Journal of the National Cancer Institute·Oliver StoeltzingLee M Ellis
Aug 17, 2004·Trends in Cell Biology·Maria E Giannakou, Linda Partridge
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Weida GongKeping Xie
Nov 6, 2008·Medicinal Research Reviews·Kenneth MaieseJinling Hou
Dec 17, 2009·Molecular and Cellular Biochemistry·Rakesh K SrivastavaSharmila Shankar
Dec 1, 2004·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Soon-Sun HongKyu-Won Kim
Jun 19, 2010·Cancer Biology & Therapy·Yanxia WangLili Liu
May 27, 2011·Cancer Biology & Therapy·Yuqing ZhangJi-hye Paik
Aug 26, 2011·International Journal of Surgical Pathology·An Ning FengJun Yang
Oct 12, 2012·Annals of the New York Academy of Sciences·Na-Young Song, Young-Joon Surh
Apr 20, 2014·World Journal of Gastroenterology : WJG·Hiroyuki YamamotoFumio Itoh
Jul 26, 2014·Journal of Gastric Cancer·Jun Haeng LeeHyun Jung Kim

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Citations

Aug 27, 2018·Journal of Cellular Biochemistry·Elham ZendedelAmirhossein Sahebkar
Feb 24, 2018·International Journal of Cancer. Journal International Du Cancer·Shuai JiangYang Yang
Mar 1, 2020·International Journal of Molecular Sciences·Chien-Lin LuYee-Yung Ng
Jan 26, 2018·Seminars in Cancer Biology·Matías González-QuirozClaudio Hetz
Mar 25, 2021·Journal of Gastrointestinal Cancer·Magdalena TyczyńskaAlicja Forma
Jan 19, 2019·Trends in Biochemical Sciences·Philippe IcardHubert Lincet

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Methods Mentioned

BETA
xenografts
xenograft
Assay
enzyme-linked immunosorbent assay
transfection

Software Mentioned

SPSS
NIH Image Analysis
GraphPad Prism

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