Forks, pincers, and triggers: the tools for nucleotide incorporation and translocation in multi-subunit RNA polymerases.

Current Opinion in Structural Biology
Dorothy A Erie, Scott R Kennedy

Abstract

The central role of RNA polymerase (RNAP) is to catalyze the processive synthesis of a growing RNA transcript. Recent structural and biophysical data have led to a deeper understanding of the nucleotide addition cycle and insight into the structure-function relationships that govern transcription elongation. In this review, we discuss kinetic data on nucleotide incorporation in the context of crystal structures, which show RNAP in multiple conformations. We present a facilitated Brownian ratchet model of nucleotide incorporation, in which templated NTP binding to a non-catalytic site in the main channel promotes the conformational changes that lead to opening of the catalytic site and translocation.

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Citations

Mar 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Scott R Kennedy, Dorothy A Erie
Jul 7, 2011·The Journal of Biological Chemistry·Maria L KireevaMikhail Kashlev
Nov 8, 2012·Transcription·Yuri A NedialkovZachary F Burton
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Matthew H LarsonSteven M Block
Jun 10, 2011·Advances in Physiology Education·Lindsay DoHarrisLuke J Janssen
Aug 28, 2013·Chemical Reviews·Robert O J Weinzierl

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