PMID: 2491754Jan 1, 1989Paper

Formaldehyde-amine fixatives for immunocytochemistry of cultured Xenopus myocytes

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
P W Luther, R J Bloch

Abstract

Although formaldehyde is commonly used in immunocytochemical studies, this fixative can cause distortions in cell structure. We tested the possibility that adducts of formaldehyde and primary amines could be used as improved fixatives for immunolabeling studies of cultured cells. A variety of primary amines were reacted with formaldehyde and applied to cultured Xenopus muscle cells, after which the cultures were labeled for immunofluorescence. Amine-formaldehyde fixatives improved structural preservation of the myocytes as compared with formaldehyde alone. The extent of improvement depended on the amine tested; the best results were obtained using cyclohexylamine. Immunofluorescence localization of a variety of antigens was better in myocytes fixed with cyclohexylamine-formaldehyde than in cells fixed with formaldehyde alone. In addition, the fixative provided good ultrastructural preservation of cytoskeletal structures and permitted immunogold labeling for alpha-actinin by use of pre-embedding labeling techniques.

Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·S J SamuelssonR J Bloch
Jan 1, 1990·Cell Motility and the Cytoskeleton·D W Pumplin, R J Bloch
Jan 1, 1991·Cell Motility and the Cytoskeleton·J A UrsittiR J Bloch
Apr 1, 1990·Journal of Electron Microscopy Technique·D W PumplinJ Strong
Oct 1, 1993·Cell and Tissue Research·M Masson-PévetP Pévet
Jan 1, 1989·Current Opinion in Immunology·J E Beesley
Jul 1, 1993·The Journal of Cell Biology·S J SamuelssonR J Bloch
Jan 12, 2010·Journal of Biomedical Optics·Yiwen SunEmma Pickwell-MacPherson
Oct 14, 1998·The Journal of Physiology·C LamontW G Wier
Feb 18, 2016·Development·Katherine PfisterPaul Skoglund
Dec 24, 1997·Annals of the New York Academy of Sciences·M Juhaszova, M P Blaustein
Apr 15, 1996·Annals of the New York Academy of Sciences·M JuhaszovaM P Blaustein
Jan 1, 1994·The American Journal of Physiology·M JuhaszovaM P Blaustein

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