Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane

Biosensors & Bioelectronics
M Ben AliClaude Martelet

Abstract

A novel formaldehyde sensitive biosensor based on bacterial formaldehyde dehydrogenase (FDH) as a bio-recognition element has been developed. The bio-recognition membrane had bi-layer architecture and consisted of FDH, cross-linked with albumin, and of the cofactor NAD at a high concentration level (first layer). The second layer was a negatively charged Nafion membrane, which prevented a leakage of negatively charged NAD molecules from the bio-membrane. As transducers, gold electrodes SiO(2)/Si/SiO(2)/Ti/Au and electrolyte-insulator-semiconductor Si/SiO(2) (EIS) structures have been used. Changes in capacitance and impedance properties of the bio-recognition membrane have been used for monitoring formaldehyde concentration in a bulk solution. It has been shown that formaldehyde can be detected within a concentration range from 1 microM to 20mM depending on the type of transduction used, with a detection limit of 1 and 100 microM for gold-based and EIS-based transducers, respectively.

Citations

Apr 17, 2008·Analytical and Bioanalytical Chemistry·F Lisdat, D Schäfer
Aug 3, 2013·Analytical and Bioanalytical Chemistry·Thanh-Thuy Nguyen-BoisseFlorence Lagarde
Mar 7, 2008·Sensors·Dorothee GrieshaberErik Reimhult
Apr 6, 2021·Advances in Colloid and Interface Science·Ashis TripathyFilipe Samuel Silva

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