PMID: 38559May 31, 1979

Formaldehyde-fluorescamine-induced fluorescence as a property of carcinoma cells

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
L Mørch-JørgensenL I Larsson

Abstract

Fluorescamine is a sensitive cytochemical probe for primary amino groups and produces an intense general fluorescence in unfixed tissue sections reflecting the ubiquitous occurrence of such groups. Following treatment with formaldehyde, most primary amino groups react to form derivatives unable to yield fluorescence with fluorescamine. Certain cell systems, however, contain amino groups which do not react with formaldehyde but display strong reactivity with fluorescamine. In formaldehyde- and fluorescamine-treated specimens such cell systems display an intense fluorescence, whereas the majority of tissue constituents are non-fluorescent. Fluorescent cell systems include certain protein- and peptide-secreting cells and a large number of different types of carcinoma cells. In some cases it appears that neoplastic transformation is necessary before the cells display formaldehyde-fluorescamine-induced fluorescence. Available data indicate that the reactive substance(s) are peptide in nature and that the production of such substance(s) may be a general property of carcinoma cells.

Related Concepts

Derivatives
Carcinomatosis
Formol
Fluorescence
Immunofluorescence Microscopy
Fluorescent stain
Carcinoma
Cell Secretion
Fluram
Formaldehyde

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.