Formation and analysis of mannosylerythritol lipids secreted by Pseudozyma aphidis

Applied Microbiology and Biotechnology
U RauS Lang

Abstract

Pseudozyma aphidis DSM 70725 was found to be a novel producer of mannosylerythritol lipids (MELs). The MELs were quantified by HPLC. Glucose as carbon source for precultivation supported growth well. By contrast, at concentrations >30 g l(-1) in preculture, subsequent MEL formation in the main culture with soybean oil as sole carbon source was reduced. The type of substrate supply considerably influenced MEL formation. High concentrations of soybean oil (80 ml l(-1)) at init favored the production process when compared to a stepwise (20 ml l(-1)) addition. Mannose or erythritol were suitable second carbon sources that enhanced the MEL yield with soybean oil as preferred primary substrate. After 10 days, a maximum yield of 75 g l(-1) was attained during shake-flask cultivation. Biofuel (rapeseed oil methyl ester) also resulted in high yields of MEL, but glucose reduced the MEL yield. Analysis by GC-MS showed that all fatty acids contained in MEL and derived from soybean oil or related methyl ester were degraded by C2-units to differing extents. The surface (water/air) and interfacial (water/hexadecane) tension of the MELs produced from different carbon sources were reduced to a minimum of 26.2 mN m(-1) and 1 mN m(-1), respectively.

References

Jun 1, 1992·Trends in Biotechnology·A Fiechter
Nov 26, 1999·Applied Microbiology and Biotechnology·H S KimY Tani
Jun 8, 2000·International Journal of Systematic and Evolutionary Microbiology·J W FellA Statzell-Tallman
Jun 16, 2000·Applied Microbiology and Biotechnology·I M BanatS S Cameotra
Feb 28, 2002·Bioscience, Biotechnology, and Biochemistry·Koji KakugawaTokichi Miyakawa
Aug 16, 2002·Current Opinion in Biotechnology·Eliora Z Ron, Eugene Rosenberg
May 15, 2003·Journal of Biomedical Materials Research. Part a·Jae Hong ImDai Kitamoto
Dec 1, 1955·Canadian Journal of Microbiology·R H HASKINSB BOOTHROYD
Oct 20, 2005·Journal of Bioscience and Bioengineering·Dai KitamotoTadaatsu Nakahara

❮ Previous
Next ❯

Citations

Feb 25, 2005·Applied Microbiology and Biotechnology·U RauS Lang
Aug 22, 2008·Journal of Industrial Microbiology & Biotechnology·Joseph Irudayaraj ArutchelviMukesh Doble
May 1, 2010·Journal of Oleo Science·Tomotake MoritaDai Kitamoto
Sep 14, 2012·Bioscience, Biotechnology, and Biochemistry·Tomotake MoritaDai Kitamoto
Mar 10, 2009·Bioscience, Biotechnology, and Biochemistry·Tomotake MoritaDai Kitamoto
Feb 18, 2014·Applied Microbiology and Biotechnology·Sophie L K W RoelantsWim Soetaert
Dec 12, 2007·Applied Microbiology and Biotechnology·Masaaki KonishiDai Kitamoto
Feb 27, 2008·Applied Microbiology and Biotechnology·Mi-Hee ChangSeong-Chool Hong
Apr 16, 2013·Applied Microbiology and Biotechnology·Tomotake MoritaDai Kitamoto
Mar 10, 2015·Journal of Oleo Science·Tomotake MoritaDai Kitamoto
Mar 15, 2015·Enzyme and Microbial Technology·Nuno Torres FariaFrederico Castelo Ferreira
May 11, 2015·Applied Microbiology and Biotechnology·Tomotake MoritaDai Kitamoto
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Masaaki KonishiDai Kitamoto
Oct 19, 2011·Chemical Society Reviews·Patrick FoleyJulie B Zimmerman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.