PMID: 6788347Jul 20, 1981

Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH

Brain Research
E SpindelR J Wurtman

Abstract

Thyrotropin-releasing hormone (TRH) injected into the lateral ventricle of the rat's brain was rapidly metabolized to deamido-TRH (DA-TRH). Brain levels of TRH decreased with a half-life of 7 min, while the DA-TRH formed from its disappeared with a half-life of 2.5 min. To prevent the post-mortem degradation of DA-TRH it was necessary to sacrifice rats by directing focused microwave irradiation towards the brain. The short half-lives determined for TRH and DA-TRH in vivo were much shorter than those obtained using in vitro techniques. The in vivo formation and accumulation of DA-TRH was inhibited by bacitracin and unaffected by probenicid.

References

Jul 1, 1979·The Journal of Clinical Endocrinology and Metabolism·C H EmersonB L Currie
Apr 1, 1975·The Journal of Clinical Endocrinology and Metabolism·T J VisserG Hennemann
Jul 1, 1975·The Journal of Clinical Endocrinology and Metabolism·G E ShambaughE R Blonsky
Sep 24, 1976·Biochimica Et Biophysica Acta·W L Taylor, J E Dixon
Nov 22, 1976·Biochemical and Biophysical Research Communications·J F McKelvyC Kordon
Mar 1, 1972·Endocrinology·R M Bassiri, R D Utiger
Jan 1, 1980·Experimental Brain Research·O JohanssonL A Sternberger

Citations

Nov 1, 1983·Molecular and Cellular Endocrinology·E C Griffiths, J R McDermott
Sep 1, 1984·Brain Research Bulletin·G K RiekeJ F Hunter
Sep 6, 2002·Physiology & Behavior·Yang-Ho ChoiClifton A Baile
Sep 1, 1984·Journal of Neurosurgery·M BernsteinJ Deck
Apr 26, 2012·The Journal of Pharmacology and Experimental Therapeutics·Ivan LazcanoJean-Louis Charli

Related Concepts

Thyrotropin-releasing hormone, deamido-
Metazoa
Altracin
Brain
Half-Life
Injections, Intraventricular
Pro-Cid
Pyroglutamate
Proterelin Tartrate Hydrate
Rats, Laboratory

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