Mar 1, 1981

Formation and fate of heterokaryons involving embryonal carcinoma cells

Somatic Cell Genetics
M Featherstone, M McBurney

Abstract

We have shown that murine embryonal carcinoma (EC) cells will readily fuse to non-EC cells following treatment with polyethylene glycol (PEG). Heterokaryons were detected by autoradiography and by the induction of premature chromosome condensation (PCC). Those heterokaryons formed between EC cells and certain of the non-EC cell types did not proliferate to form viable hybrids. For example, hybrids were not recovered from EC/HeLa heterokaryons in spite of the fact that both EC/3T3 and HeLa/3T3 heterokaryons gave rise to hybrids at high frequency. No hybrids were recovered from fusion mixtures involving Chinese hamster (CHO) cells and EC; however, if the differentiated progeny of the EC cells were used, hybrids were recovered. These results suggest that both developmental and phylogenetic differences between parental cells may contribute to the ability of heterokaryons to proliferate indefinitely.

  • References24
  • Citations7

Mentioned in this Paper

Cricetulus
Chinese Hamster
Mice, Inbred BALB C
Murine
Ovary
Kidney
Embryonal Carcinoma
Cell Differentiation Process
Embryonal Carcinoma Stem Cells
Hybrids

About this Paper

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.