Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes.

American Journal of Physiology. Heart and Circulatory Physiology
Su-Jie JiaPin-Lan Li

Abstract

CD38 contains an ADP ribosylcyclase domain that mediates intracellular Ca(2+) signaling by the production of cyclic ADP-ribose (cADPR), but the mechanisms by which the agonists activate this enzyme remain unclear. The present study tested a hypothesis that a special lipid-raft (LR) form, ceramide-enriched lipid platform, contributes to CD38 activation to produce cADPR in response to muscarinic type 1 (M(1)) receptor stimulation in bovine coronary arterial myocytes (CAMs). By confocal microscopic analysis, oxotremorine (Oxo), an M(1) receptor agonist, was found to increase LR clustering on the membrane with the formation of a complex of CD38 and LR components such as GM(1), acid sphingomyelinase (ASMase), and ceramide, a typical ceramide-enriched macrodomain. At 80 microM, Oxo increased LR clustering by 78.8%, which was abolished by LR disruptors, methyl-beta-cyclodextrin (MCD), or filipin. With the use of a fluorescence resonance energy transfer (FRET) technique, 15.5+/-1.9% energy transfer rate (vs. 5.3+/-0.9% of control) between CD38 and LR component, ganglioside M(1) was detected, further confirming the proximity of both molecules. In the presence of MCD or filipin, there were no FRET signals detected. In floated detergent-r...Continue Reading

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Citations

Feb 8, 2011·Antioxidants & Redox Signaling·Si JinPin-Lan Li
Jan 30, 2009·Cardiovascular Research·Catherine Pavoine, Françoise Pecker
Jun 17, 2010·The Journal of Pharmacology and Experimental Therapeutics·Lynn C HullWilliam L Dewey
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Cai-Xia LiPin-Lan Li
Feb 25, 2010·FEBS Letters·Branka Stancevic, Richard Kolesnick
Oct 6, 2014·Current Opinion in Immunology·Hajime KonoEicke Latz
Dec 29, 2009·Biochimica Et Biophysica Acta·Chun ZhangPin-Lan Li
Aug 1, 2014·American Journal of Respiratory Cell and Molecular Biology·Suengwon LeeJames S K Sham
Sep 18, 2014·Frontiers in Physiology·Nadine BeckmannBärbel Edelmann
Jun 10, 2010·Free Radical Research·Chun Zhang, Pin-Lan Li

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