PMID: 7526174Nov 1, 1994Paper

Formation and stability of acetaldehyde-induced crosslinks between poly-lysine and poly-deoxyguanosine

Mutation Research
J R Kuykendall, Matthew S Bogdanffy

Abstract

The amino acid residue and nucleoside specificity of acetaldehyde-induced DNA-protein crosslinks (DPXLs) were studied using a modified filter binding assay. A 40% inhibition of acetaldehyde-induced pUC13 plasmid DNA-calf thymus histone crosslink formation was achieved by addition of 50 mM lysine (free base), while arginine was unable to affect crosslink formation at concentrations to 150 mM. Polymers (5-mers) of lysine (poly-lys5) were able to substitute for histones in acetaldehyde-induced plasmid crosslink formation, being equally effective at equimolar concentrations. Homopolymers (6-mers) of deoxyguanosine (poly-dG6) (but not deoxyadenosine, deoxycytidine or thymidine) served as an efficient substrate for acetaldehyde-induced DPXL formation, using either calf thymus histones or poly-lys5 as the protein source. Acetaldehyde-induced crosslinks between poly-dG6 and poly-lys5 were formed rapidly, but were unstable at 37 degrees C (a half-life or 1.5-2 h). Stability of these crosslinks was unaffected by pH at a range of 5.5-9.0 at 37 degrees C for 2 h. Results presented here suggest that unstable complexes of deoxyguanosine and lysine constitute a major portion of the DPXLs formed by acetaldehyde in vitro.

References

Jan 1, 1977·Mutation Research·C AuerbachJ Moutschen
Jan 23, 1976·The Journal of Organic Chemistry·R C Moschel, N J Leonard
Nov 1, 1992·Carcinogenesis·J R Kuykendall, M S Bogdanffy
Nov 1, 1990·Journal of Cellular Biochemistry·M Costa
Aug 1, 1983·Toxicology and Applied Pharmacology·M Casanova-Schmitz, H D Heck
Sep 1, 1984·Archives of Toxicology·K Hemminki, R Suni
Oct 1, 1981·Toxicology Letters·K Hemminki
Aug 1, 1948·Journal of the American Chemical Society·H FRAENKEL-CONRAT, H S OLCOTT

❮ Previous
Next ❯

Citations

Jul 10, 1996·Mutation Research·M CostaM Bogdanffy
Aug 16, 2001·Nucleic Acids Research·N Murata-Kamiya, H Kamiya
Feb 22, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Benjamin C MoellerJames A Swenberg
Nov 5, 2002·Annual Review of Pharmacology and Toxicology·J G HengstlerF Oesch
Jun 10, 2014·Chemical Research in Toxicology·Richard M LoPachin, Terrence Gavin
Sep 18, 2007·Mutation Research·Irina G MinkoR Stephen Lloyd
Aug 11, 2005·Analytical Biochemistry·Sharon BarkerMichael Weinfeld
Aug 2, 2005·Alcohol·Ramona G Dumitrescu, Peter G Shields
Aug 30, 2013·Critical Reviews in Toxicology·Richard J Albertini
Feb 14, 2007·Alcoholism, Clinical and Experimental Research·Kala VisvanathanKathy J Helzlsouer
Nov 4, 2017·Science China. Life Sciences·Naeh L Klages-Mundt, Lei Li
Mar 21, 2021·Applied Microbiology and Biotechnology·Lahiru N Jayakody, Yong-Su Jin
Aug 12, 2008·Chemical Research in Toxicology·Laurie A VanderVeenLawrence J Marnett
Feb 23, 2005·Biochemistry·Irina G MinkoR Stephen Lloyd

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.