PMID: 3745125Sep 1, 1986Paper

Formation in Rhizobium and Agrobacterium spp. of a 235-kilodalton protein intermediate in beta-D(1-2) glucan synthesis

Journal of Bacteriology
A Zorreguieta, R A Ugalde

Abstract

beta-D(1-2) Glucan was synthesized by Agrobacterium and Rhizobium spp. in vitro with enzymes from the internal membranes upon the addition of UDF glucose and Mg2+ or Mn2+. An intermediate containing protein and beta-D(1-2) glucan was formed during the reaction. It could be precipitated with trichloroacetic acid or separated by polyacrylamide gel electrophoresis under denaturing conditions. After detection with Coomassie blue or a radioactive substrate, the intermediate appeared as a 235-kilodalton protein. The radioactivity could be chased with a nonradioactive substrate. All strains that formed beta-D(1-2) glucan in vitro formed the 235-kilodalton protein, whereas avirulent, beta-D(1-2) glucan-negative mutants did not synthesize it. Transposon insertions in the chvB locus of strains ME2 and ME116 did not alter the virulence of the strains. These strains were able to form beta-D(1-2) glucan in vitro and synthesize the 235-kilodalton protein.

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Citations

Apr 7, 2018·Cellular Microbiology·Leticia S GuidolinJean-Pierre Gorvel
May 1, 1992·Plant Molecular Biology·P J Hooykaas, R A Schilperoort
Oct 6, 2000·Annual Review of Microbiology·H P Spaink
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Jul 1, 1996·World Journal of Microbiology & Biotechnology·K Weising, G Kahl
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Jul 6, 2000·Microbiology·N I de IanninoR A Ugalde
Apr 1, 1997·Microbiology·Gabriel BrionesRodolfo A Ugalde
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Mar 1, 1992·Microbiological Reviews·S C Winans
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Aug 1, 1990·Journal of Bacteriology·A ZorreguietaR A Ugalde
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Jul 1, 1991·Journal of Bacteriology·J L Cohen, K J Miller
Jun 1, 1994·Microbiological Reviews·M W Breedveld, K J Miller

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