Formation mechanism of the internal structure of type I protein bodies in rice endosperm: relationship between the localization of prolamin species and the expression of individual genes

The Plant Journal : for Cell and Molecular Biology
Yuhi SaitoTakehiro Masumura

Abstract

Rice prolamins, a group of seed storage proteins, are synthesized on the rough endoplasmic reticulum (ER) and form type I protein bodies (PB-Is) in endosperm cells. Rice prolamins are encoded by a multigene family. In this study, the spatial accumulation patterns of various prolamin species in rice endosperm cells were investigated to determine the mechanism of formation of the internal structure of PB-Is. Immunofluorescence microscopic analysis of mature endosperm cells showed that the 10 kDa prolamin is mainly localized in the core of the PB-Is, the 13b prolamin is localized in the inner layer surrounding the core and the outermost layer, and the 13a and 16 kDa prolamins are localized in the middle layer. Real-time RT-PCR analysis showed that expression of the mRNA for 10 kDa prolamin precedes expression of 13a, 13b-1 and 16 kDa prolamin in the developing stages. mRNA expression for 13b-2 prolamin occurred after that of the other prolamin species. Immunoelectron microscopy of developing seeds showed that the 10 kDa prolamin polypeptide initially accumulates in the ER, and then 13b, 13a, 16 kDa and 13b prolamins are stacked in layers within the ER. Studies with transgenic rice seeds expressing prolamin-GFP fusion proteins unde...Continue Reading

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Citations

Aug 24, 2013·International Journal of Molecular Sciences·Hyun-Jung KimYoung-Mi Kim
Oct 10, 2014·Journal of Nutritional Science and Vitaminology·Masatoshi KubotaMotoni Kadowaki
Jul 3, 2015·International Journal of Molecular Sciences·Hye-Jung LeeYoung-Mi Kim
Jan 19, 2016·Journal of Bioscience and Bioengineering·Masaki OkudaTakuro Ishii
Jun 24, 2015·Plant Biotechnology Journal·Fumio TakaiwaTakachika Hiroi
Aug 20, 2016·Frontiers in Plant Science·Emanuela PedrazziniAlessandro Vitale
Dec 29, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Richard E BoylesStephen Kresovich
Nov 22, 2017·International Journal of Molecular Sciences·Yeong-Min JoJong-Yeol Lee
Dec 7, 2018·Journal of Experimental Botany·Hiroshi WadaHiroshi Nakano
Apr 28, 2019·Nature Communications·Yihao YangChangjie Yan
Nov 16, 2016·Frontiers in Plant Science·Kyoungwon ChoYoung-Mi Kim
Sep 29, 2021·Journal of Integrative Plant Biology·Wei HeFeng Yu

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