Formation of 1:1 complex of the cytokine receptor homologous region of granulocyte colony-stimulating factor receptor with ligand

Bioscience, Biotechnology, and Biochemistry
O HiraokaY Ota

Abstract

The cytokine receptor homologous (CRH) region of the murine granulocyte colony-stimulating factor (G-CSF) receptor was secreted using a Escherichia coli maltose binding protein (MBP) fusion system. The CRH region was prepared from the periplasmic fraction by G-CSF affinity column chromatography and restriction protease factor Xa digestion, and was purified to homogeneity. The purified CRH region specifically bound G-CSF, with an apparent dissociation constant (kd) of about 1.5 x 10(-9) M. A 1:1 CRH.G-CSF complex was established by gel-filtration high pressure liquid chromatography (HPLC). However, a 2:1 stoichiometric complex was not established, as in the case of the growth hormone (GH) receptor [Recent Prog. Hormone Res., 48, 233-275 (1993)].

Citations

Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Taro TamadaRyota Kuroki
May 6, 2004·Science's STKE : Signal Transduction Knowledge Environment·Robert M Stroud, James A Wells

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