PMID: 6112746Mar 1, 1981Paper

Formation of 11-trans slow reacting substances

Proceedings of the National Academy of Sciences of the United States of America
V AtracheC J Sih

Abstract

Under strongly basic conditions [excess LiOH, dimethoxyethane/water (4:1, vol/vol)], purified slow reacting substances (SRSs) SRS-GSH and SRS-Cys were not isomerized to their corresponding 11-trans isomers. However, addition of thiols such as glutathione (GSH) or L-cysteine to this basic medium produced various amounts of 11-trans-SRS, depending on the thiol concentration. This chemical isomerization was inhibited by the radical scavenger 4-hydroxy-2,2,6,6-tetramethylpiperidinooxy free radical (HTMP); the inhibition suggests that the thiyl radical (RS) is added reversibly to the triene system at C-12, resulting in the overall cis leads to trans isomerization of the 11,12 double bond. Because the amount of 11-trans-SRS-Cys produced by intact rat basophilic leukemia (RBL-1) cells was consistently higher than the amount produced in boiled cells, we believe that intact RBL-1 cells contain enzyme systems that form peroxides, which are known to enhance the formation of thiyl radicals, required for cis leads to trans isomerization. Likewise, HTMP inhibited the formation of 11-trans-SRS-Cys in this cell system.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R C MurphyB Samuelsson
Dec 28, 1979·Biochemical and Biophysical Research Communications·S HammarströmE J Corey
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L OrningB Samuelsson
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A LewisE J Corey
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J HouglumC J Sih
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D E SokC J Sih
Jun 30, 1980·Biochemical and Biophysical Research Communications·D A ClarkB Samuelsson

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Citations

Jul 16, 1981·Biochemical and Biophysical Research Communications·D E SokC J Sih

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