Formation of a molten-globule-like state of myoglobin in aqueous hexafluoroisopropanol

Biochemical and Biophysical Research Communications
J R Cort, Niels H Andersen

Abstract

The effects of aqueous hexafluoroisopropanol (HFIP) media on the structure of myoglobin are reported. Circular dichroism (CD) spectra of this alpha-helical protein in as little as 4% (v/v) HFIP indicate that native-like amounts of secondary structure remain while rigid tertiary structure is lost. However, thermal studies suggest some residual cooperativity of unfolding in this state. At much higher HFIP concentrations, the helicity exceeds the native value and the protein behaves as a series of independent helices which do not interact with each other. We did not observe cold denaturation of myoglobin, even though this phenomenon has been observed for molten globule states of myoglobin, as well as for monomeric amphipathic alpha-helices when moderate quantities of HFIP are present. The pH dependence of trifluoroethanol-induced disruption of tertiary structure revealed that the degree of disruption increases as the enthalpic advantage of the folded state is diminished at low pH.

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Citations

Jul 15, 2009·Protein Engineering, Design & Selection : PEDS·John R CortNiels H Andersen
Mar 18, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Carol B FowlerJeffrey T Mason
Jan 27, 2010·Biotechnology Advances·Nandana Bhardwaj, Subhas C Kundu
Jul 8, 2010·Journal of Biomedical Materials Research. Part a·Ting LiuSing Yian Chew
Mar 16, 2016·Journal of the American Chemical Society·Alex P S Brogan, Jason P Hallett
Mar 4, 2008·Biomaterials·Dimitrios I ZeugolisMichael Raghunath
Sep 26, 2002·Journal of Biochemistry and Molecular Biology·Suman KunduMedicherla V Jagannadham
Feb 11, 2015·Journal of Photochemistry and Photobiology. B, Biology·Farangis Ataei, Saman Hosseinkhani
Jul 4, 2012·International Journal of Biological Macromolecules·Ganesh ShanmugamAsit Baran Mandal
Jun 24, 1998·Journal of Molecular Biology·Y Luo, R L Baldwin
Jul 4, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Milan R MilovanovićJean-Pierre Djukic
Nov 3, 2004·Archives of Biochemistry and Biophysics·Aabgeena NaeemRizwan Hasan Khan
Apr 21, 2005·Biochimica Et Biophysica Acta·Farah Naseem, Rizwan Hasan Khan
Jul 19, 2005·International Journal of Biological Macromolecules·Soghra Khatun Haq, Rizwan Hasan Khan

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