Formation of a novel arachidonic acid metabolite in peroxisomes

Prostaglandins, Leukotrienes, and Essential Fatty Acids
J A GordonA A Spector

Abstract

A new radiolabeled metabolite was released into the extracellular fluid by normal human skin fibroblasts that were labeled with [5,6,8,9,11,12,14,15-3H] arachidonic acid. This product continued to accumulate during a 24 h incubation, and its formation was not saturated at arachidonic acid concentrations up to 15 mumol/L. The compound, identified as hexadecatrienoic acid, was not produced by Zellweger fibroblasts which are deficient in peroxisomal fatty acid beta-oxidation. By contrast, radiolabeled hexadecatrienoic acid was produced by mutant fibroblasts having other peroxisomal defects, including X-linked adrenoleukodystrophy, adult Refsum's disease, and rhizomelic chondrodysplasia punctata. This radiolabeled metabolite also was produced by mutant fibroblasts that cannot oxidize long-chain fatty acids in the mitochondria. These results indicate that hexadecatrienoic acid is synthesized from arachidonic acid by peroxisomal beta-oxidation. The absence of this pathway may account for some of the biochemical and functional abnormalities that occur in Zellweger's syndrome.

References

Jan 1, 1991·Clinical Genetics·A PoulosG Wise
Aug 1, 1991·Clinical Biochemistry·W J Rhead
Jan 1, 1989·Progress in Lipid Research·A Poulos
Dec 5, 1986·Biochimica Et Biophysica Acta·E ChristensenB O Christophersen
Jan 1, 1983·Journal of Inherited Metabolic Disease·R I Kelley, B E Corkey
Jun 1, 1983·Pediatric Research·J M TrijbelsM van den Broek-Van Essen
Jul 1, 1993·The Journal of Clinical Investigation·J A GordonA A Spector

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Citations

Sep 2, 1999·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A A SpectorR L Widstrom
Aug 22, 2001·Biochimica Et Biophysica Acta·K De NysP P Van Veldhoven
Jul 1, 1997·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A A SpectorJ A Gordon

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