PMID: 8441655Feb 11, 1993Paper

Formation of AraC-DNA sandwiches

Nucleic Acids Research
J H Carra, R F Schleif

Abstract

With the use of special DNA binding sites, but not the natural aral binding site, the dimeric AraC protein can be forced to make sandwich structures in which two DNA molecules are joined by two AraC protein dimers. Apparently one subunit from each dimer contacts each DNA molecule in an extended structure. These sandwich structures form only in the absence of arabinose. This behavior is consistent with the protein's ability to form DNA loops by binding to separated half sites in the absence of arabinose and its preference for binding to adjacent half-sites in the presence of arabinose.

References

Apr 1, 1990·The EMBO Journal·S OehlerB Müller-Hill
Oct 26, 1990·Science·R B Lobell, R F Schleif
Oct 20, 1989·Journal of Molecular Biology·A Brunelle, R Schleif
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L HuoR Schleif
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K MartinR F Schleif
Jul 5, 1987·Journal of Molecular Biology·J A BorowiecJ D Gralla
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W Hendrickson, R Schleif
Sep 25, 1984·Journal of Molecular Biology·W Hendrickson, R F Schleif

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Citations

Jun 20, 1998·Journal of Molecular Biology·R R Seabold, R F Schleif

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