PMID: 8600986Mar 15, 1996Paper

Formation of beta-glucuronides and of beta-galacturonides of various retinoids catalyzed by induced and noninduced microsomal UDP-glucuronosyltransferases of rat liver

Biochimica Et Biophysica Acta
G GenchiJ A Olson

Abstract

The complex of microsomal UDP-glucuronosyl transferases (UGT; EC 2.4.1.17) of rat liver catalyzes the formation of retinoyl beta-glucuronide (RAG) from retinoic acid (RA) and retinyl beta-glucuronide (ROG) from retinol (ROL) in the presence of UDP-glucuronic acid (UDPGA). The relative rates of formation of beta-glucuronides in noninduced microsomes were: 9-cis RA > 13-cis RA > all-trans 4-oxo RA > TTNPB > all-trans RA > CD-367 > 13-cis ROL > 9-cis ROL > acitretin > all-trans ROL. Michaelis constants (KM) for all-trans RA, 13-cis ROL, TTNPB and UDPGA were 130 microM, 300 microM, 210 microM and 2.6 microM, respectively. Galacturonides of RA, but not detectably of ROL, were formed from UDP-galacturonic acid at 11-30% the rate of the beta-glucuronides, whereas UDP derivatives of nonionized sugars did not serve as substrates. Pretreatment of rats with 3-methylcholanthrene markedly increased RAG formation in the absence of detergent (Triton X-100), but less so in its presence. Clofibrate pretreatment doubled the rate of RAG formation, whereas phenobarbital pretreatment showed little effect. N-Ethylmaleimide (5 microM) minimized cis-trans isomerization without significantly affecting glucuronidation. Rates of glucuronidation of retino...Continue Reading

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Citations

May 29, 1998·Brain Research. Developmental Brain Research·M YamamotoP McCaffery
Aug 15, 2009·PPAR Research·Melissa Runge-Morris, Thomas A Kocarek
Sep 3, 2009·Journal of Lipid Research·Renate SchreiberAchim Lass
Nov 21, 2012·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·C M Gliddon, R J Rosengren
Jan 1, 2003·Biochemical and Biophysical Research Communications·Andreas J Kesel
Oct 6, 2009·The International Journal of Biochemistry & Cell Biology·Erika CioneGiuseppe Genchi
Nov 1, 2008·Drug Metabolism and Pharmacokinetics·Yuan LuAnna Radominska-Pandya
Oct 11, 2011·Biochimica Et Biophysica Acta·Maureen A Kane
Jul 5, 2001·Toxicology and Applied Pharmacology·B J Bray, R J Rosengren
Jul 2, 2002·Journal of Lipid Research·James V ChithalenGlenville Jones
May 26, 2018·Journal of Neural Engineering·Siliang WuTreena Livingston Arinzeh
Jul 14, 2000·Physiological Reviews·S A RossL M De Luca
Aug 17, 2019·Pharmacology & Therapeutics·Nina Isoherranen, Guo Zhong
Jun 11, 2002·Archives of Biochemistry and Biophysics·Yuanping WangA Catharine Ross

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