Formation of biofilm at different nutrient levels by various genotypes of Listeria monocytogenes

Journal of Food Protection
James P FolsomJ F Frank

Abstract

Strains of Listeria monocytogenes differ in their ability to form biofilms. The objectives of this study were to determine whether genetically related strains have similar biofilm-forming capacities and what effect nutrient concentration has on the ability of different strains to produce biofilms. Biofilms of 30 strains of L. monocytogenes, obtained from a variety of sources were grown on stainless steel in tryptic soy broth (TSB) or in a 1:10 dilution of TSB (DTSB) for 24 h at 32 degrees C. The amount of biofilm formed was determined with image analysis after cells were stained with bisBenzimide H 33258 (Hoechst 33258). The strains were genetically subtyped by repetitive element sequence-based PCR (rep-PCR) with the primer set rep-PRODt and rep-PROG5. Data were analyzed with an analysis of variance and Duncan's multiple range test. Eleven strains produced the same amount of biofilm in both media. Fourteen strains produced more biofilm in TSB than in DTSB. Five strains produced more biofilm in DTSB than in TSB. Serotype 4b strains produced more biofilm in TSB than did serotype 1/2a strains, whereas serotype 1/2a strains produced more biofilm in DTSB than did serotype 4b strains. Growth in DTSB resulted in decreased biofilm accu...Continue Reading

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Citations

Aug 18, 2009·Journal of Food Protection·Hajime TakahashiBon Kimura
Jun 19, 2013·Critical Reviews in Food Science and Nutrition·Wladir B Valderrama, Catherine N Cutter
Jan 7, 2016·Frontiers in Microbiology·Marion ZetzmannChristian U Riedel
Aug 4, 2009·Applied and Environmental Microbiology·Youwen PanSophia Kathariou
Oct 9, 2007·Applied and Environmental Microbiology·Frédéric AuvrayBrigitte Carpentier
Feb 4, 2014·International Journal of Microbiology·Joana BarbosaPaula Teixeira
May 16, 2014·Foodborne Pathogens and Disease·Moutong ChenXiaobing Yang
Oct 25, 2017·Environmental Science and Pollution Research International·Dugeshwar KarleyToleti Subba Rao
Aug 4, 2012·Journal of Food Protection·António LourençoJoseph F Frank
Apr 4, 2015·Journal of Food Protection·Michael C YeaterWesley N Osburn
Nov 17, 2017·The Journal of General and Applied Microbiology·Sumreen HayatTimothy D McHugh
Nov 30, 2019·International Journal of Food Microbiology·Lionel Kenneth DygicoCatherine M Burgess
Oct 5, 2011·International Journal of Food Microbiology·Julie SzlavikDennis S Nielsen

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