PMID: 8610152Apr 2, 1996Paper

Formation of chimeric DNA primer extension products by template switching onto an annealed downstream oligonucleotide

Proceedings of the National Academy of Sciences of the United States of America
R PatelE F Ullman

Abstract

Oligodeoxynucleotide sequences are described that anneal to a template downstream of a priming site. During polymerase-catalyzed extension of the primer, the extending primer shifts from the original template to a segment of the annealed oligonucleotide that acts as an alternative template. The resulting chimeric extended primer has one segment that is complementary to the template and a second segment that is complementary to the oligonucleotide. The influence of the sequence elements of the oligonucleotide and the reaction conditions on template switching have been explored. The sequence requirements for template switching are compared to those for transposon excision.

References

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Citations

Feb 13, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·F V ShammasA Osland
Oct 20, 2005·Journal of Bioscience and Bioengineering·Takahiro Kanagawa
Dec 7, 2002·The Plant Cell·Allen SessionsStephen A Goff
Dec 3, 2004·Applied and Environmental Microbiology·Shinya KurataYoichi Kamagata
Feb 5, 2009·BMC Research Notes·Fotini Ieremiadou, George C Rodakis
Oct 23, 2012·Virology·Melissa B Duhaime, Matthew B Sullivan
May 5, 2018·Nature Protocols·Peter ChovanecAnne E Corcoran
Apr 2, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Hainer WackerbarthJens Ulstrup

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