Formation of circular satellite tobacco ringspot virus RNA in protoplasts transiently expressing the linear RNA

Virology
C A ChayG Bruening

Abstract

The most abundant form of the satellite RNA of tobacco ringspot virus (sTRSV RNA) is a linear, unit length molecule of 359 nucleotide residues, designated L-(+)M. A postulated replication scheme for the satellite RNA has as its first, and apparently virus-independent, step the ligation of L-(+)M into the corresponding circular form C-(+)M. We transiently expressed L-(+)M wild type and L-(+)M mutants in tobacco protoplasts using an African cassava mosaic geminivirus vector. Measured extents of C-(+)M accumulation were correlated with computer-predicted folding to suggest wild-type secondary structure elements that might be deleted without reducing ligation. A 127-nucleotide residue mutant L-(+)M was created by replacing, with 7 and 3 residues, respectively, nucleotide residues 53-211 and 268-350, each of which was predicted to form a set of three adjacent imperfect stem-loops in wild-type L-(+)M. The mutant L-(+)M was found to be extensively ligated to C-(+)M in protoplasts and to retain a calculated helix of the wild-type molecule that incorporates the 3' terminal sequence. A trinucleotide in the 3' region was mutated so as to disrupt and restore, respectively, the calculated helix, reducing and restoring, respectively, C-(+)M ...Continue Reading

References

Jul 1, 1977·Virology·R A Owens, I R Schneider
Jan 1, 1992·Annual Review of Biochemistry·R H Symons
Jun 25, 1992·Nucleic Acids Research·K J HertelR Hodgson
May 1, 1991·Molecular Plant-microbe Interactions : MPMI·G BrueningP A Feldstein
Apr 25, 1991·Nucleic Acids Research·B L Apostol, C L Greer
May 11, 1988·Nucleic Acids Research·J M BuzayanG Bruening
Jul 15, 1988·Biochemical and Biophysical Research Communications·J M KaperG Steger
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M KonarskaH J Gross
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P DavanlooF W Studier
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R PerrimanW J Peacock
Mar 28, 1986·Science·G A ProdyG Bruening

❮ Previous
Next ❯

Citations

Jan 7, 2006·Oligonucleotides·Alexander V VlassovSergei A Kazakov
Mar 3, 2004·Journal of Virology·Sang Ik Song, W Allen Miller
May 7, 2009·Annual Review of Biophysics·Martha J Fedor
Jun 7, 2011·Annual Review of Phytopathology·George Bruening
Nov 20, 2004·Virus Genes·Hideki Horiuchi, Toshiyuki Fukuhara
Jan 11, 2003·Biotechnology & Genetic Engineering Reviews·Claudine Porta, George P Lomonossoff
Mar 15, 2000·Journal of Molecular Biology·M J Fedor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.