Formation of deglycosylated alpha-L-fucosidase by endo-beta-N-acetylglucosaminidase in Fusarium oxysporum.

Applied and Environmental Microbiology
Y TsujiT Tochikura

Abstract

Two forms of alpha-L-fucosidase, deglycosylated and glycosylated, were found in the fucose-inducing culture broth of Fusarium oxysporum. Endo-beta-N-acetylglucosaminidase was also found in the same culture broth. The deglycosylated alpha-L-fucosidase was purified from the culture broth to homogeneity on polyacrylamide disc gel electrophoresis and analytical ultracentrifugation. Purified deglycosylated alpha-L-fucosidase was compared in chemical composition and immunological homology with glycosylated alpha-L-fucosidase which had been reported previously (K. Yamamoto, Y. Tsuji, H. Kumagai, and T. Tochikura, Agric. Biol. Chem. 50: 1689, 1986). Both enzymes had nearly the same amino acid compositions and were immunologically identical. Glycosylated alpha-L-fucosidase had mannose, galactose, and N-acetylglucosamine residues. In contrast, the deglycosylated enzyme had only N-acetylglucosamine residues. These results suggest that the deglycosylated alpha-L-fucosidase is formed by the release of sugar chains from the glycosylated form by Fusarium endo-beta-N-acetylglucosaminidase. Furthermore, various enzymatic properties were compared: the two alpha-L-fucosidases were found to exhibit similar catalytic activities and thermal stabilit...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J P PrieelsR L Hill
Aug 29, 1980·Biochemical and Biophysical Research Communications·F S FurbishR O Brady
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Jan 1, 1946·The Biochemical Journal·T W Goodwin, R A Morton
Jan 1, 1949·Acta Pathologica Et Microbiologica Scandinavica·O OUCHTERLONY
Mar 20, 1947·The Journal of General Physiology·M Kunitz

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Citations

Nov 12, 2005·Current Genetics·Vincent PhalipJean-Marc Jeltsch
Jun 27, 2009·The Journal of Microbiology·Alexandre Favarin SomeraJoão Atílio Jorge
May 1, 1991·Applied and Environmental Microbiology·C RiouM Fevre
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S W Johnson, J A Alhadeff

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