Formation of dimethylarsinic acid from methylation of sodium arsenite in lumbricus terrestris

Toxicology in Vitro : an International Journal Published in Association with BIBRA
K W LinR F Toia

Abstract

Arsenic is ubiquitous in the environment and the toxicological response of various organisms to it is dependent on the particular chemical form involved. In general, methylation of inorganic arsenic is considered to be a detoxification reaction. While this transformation is known to be mediated by methyltransferases in several species of mammals, less is known about the fate of arsenic in invertebrates. As part of a continuing interest in heavy metals and metalloid toxicology, the alkylating activity of cytosol prepared from the common earthworm, Lumbricus terrestris, towards sodium arsenite has now been investigated. Thus, S-adenosyl-l-[(14)C]methionine ([(14)C-methyl]SAM) fortified earthworm cytosol was incubated with sodium arsenite at 37 degrees C for 90 min. Initial TLC analysis of the incubation mixture suggested incorporation of radiolabel into dimethylarsinic acid. This was subsequently proven by isolation of the metabolite through radiodilution followed by recrystallization of the recovered material to constant specific activity. This result suggests that earthworm cytosol has the same methylating reactivity towards arsenite as do similar preparations from various tissues of several species of mammals.

References

Jul 1, 1990·Toxicology·B GeorisR Lauwerys
Jun 1, 1985·Archives of Toxicology·M Vahter, E Marafante
Jun 9, 1995·Journal of Chromatography. B, Biomedical Applications·M StýbloD J Thomas

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Citations

May 2, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A Furst
Nov 7, 2006·Environment International·Juliane Ventura-LimaJosé M Monserrat

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