PMID: 9528690Apr 7, 1998Paper

Formation of DNA adducts in human buccal epithelial cells exposed to acetaldehyde and methylglyoxal in vitro

Chemico-biological Interactions
C E VacaR C Grafström

Abstract

Acetaldehyde (AA) and methylglyoxal (MG) are reactive, ubiquitous aldehydes, present in the environment and endogenously formed in animals and humans. They have both been shown to readily form DNA adducts under simulated physiological conditions. We report here on the use of cultured normal and SV40T antigen-immortalized human buccal epithelial cells as model systems for aldehyde exposure of the oral epithelium, occurring through the ingestion of alcoholic beverages and brewed coffee, as well as by inhalation of tobacco smoke and automobile exhaust. By the application of recently developed 32P-postlabeling methods, the presence of both endogenous and induced AA and MG DNA adducts was demonstrated in cultured human epithelial cells. Furthermore, these DNA adducts were formed in a dose-dependent manner at aldehyde concentrations that were relatively nontoxic to the cells.

References


❮ Previous
Next ❯

Citations

Mar 5, 2003·Journal of Chromatography. a·Shinsuke InagakiMasashi Goto
Aug 15, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C J Smith, C Hansch
Jul 25, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J BlasiakM Wojewódzka
Jan 29, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H LovschallD Arenholt-Bindslev
Feb 22, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Benjamin C MoellerJames A Swenberg
Apr 6, 2001·Alcoholism, Clinical and Experimental Research·S VäkeväinenM Salaspuro
Jun 19, 2001·Alcoholism, Clinical and Experimental Research·S VäkeväinenM Salaspuro
Jun 21, 2003·Journal of Periodontology·Paola PoggiMassimo T Rota
Jun 11, 2009·Digestive Diseases and Sciences·K IijimaT Shimosegawa
Jan 30, 2016·Oxidative Medicine and Cellular Longevity·Zhiqi WangDeliang Cao
Jan 1, 2008·Chemico-biological Interactions·Miklós Péter Kalapos
Feb 6, 2008·Basic & Clinical Pharmacology & Toxicology·Ivan KosalecNevenka Kopjar
Jan 25, 2008·The FEBS Journal·Viola BreyerMonika Pischetsrieder
Jan 22, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Maria Emília Santos Pereira RamosCláudia Pessoa
Jul 6, 2010·International Journal of Radiation Biology·Jorge E GonzálezLaurence Roy
Aug 30, 2013·Critical Reviews in Toxicology·Richard J Albertini
Mar 6, 2008·American Journal of Human Genetics·Avanti Kulkarni, David M Wilson
May 1, 2016·Alcoholism, Clinical and Experimental Research·Kevin D ShieldJürgen Rehm
Jun 9, 2016·Gaceta sanitaria·José Antonio García-LavandeiraJuan Miguel Barros-Dios
Jun 23, 2016·Nutrition Reviews·Lieselot Y Hemeryck, Lynn Vanhaecke
Feb 4, 2006·The Lancet Oncology·Paolo Boffetta, Mia Hashibe
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Ville SalaspuroMikko Salaspuro
Nov 16, 2017·European Journal of Public Health·José A García LavandeiraJuan M Barros-Dios
Jul 9, 2004·International Journal of Cancer. Journal International Du Cancer·Ville Salaspuro, Mikko Salaspuro
Apr 27, 2002·Annals of the New York Academy of Sciences·John W Baynes
Jan 18, 2005·Alternatives to Laboratory Animals : ATLA·Claudia A StaabRoland C Grafström
Jun 17, 2020·Archives of Toxicology·Andrea HartwigGerhard Eisenbrand
Dec 3, 2017·Best Practice & Research. Clinical Gastroenterology·Mikko Salaspuro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.