PMID: 11933233Apr 5, 2002Paper

Formation of DNA triple helices by an oligonucleotide conjugated to a fluorescent ruthenium complex

Chembiochem : a European Journal of Chemical Biology
Günther N GrimmClaude Hélène

Abstract

A conjugate of a triple helix forming oligonucleotide (TFO) and the Lambda and Delta enantiomers of the ruthenium diphenanthroline dipyridophenazine complex [Ru(phen)(2)dppz](2+) was synthesized. The ruthenium complex was attached to the 5'-end of the TFO through the dppz moiety. This conjugate formed a stable triple helix with the polypurine tract (PPT) sequence from HIV proviral DNA. The thermal denaturation temperature of the triplex was increased by 12 degrees C. One remarkable property of the Delta-[Ru(phen)(2)dppz](2+) complex is a strong increase in its fluorescence when it intercalates into DNA. While the fluorescence of the oligonucleotide conjugate was very weak, the formation of a duplex with a complementary sequence or of a triple helix with a target duplex resulted in a large increase in fluorescence of the Delta enantiomer. The increase in fluorescence allowed us to follow the kinetics of duplex and triplex formation by fluorescence spectrometry. In contrast, the Lambda enantiomer gave a much smaller fluorescence change when a triplex was formed, even though the stability of the triplex was comparable to that of the Delta enantiomer. The property was ascribed to intercalation of the dipyridophenazine moiety of the...Continue Reading

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Citations

Feb 9, 2013·Chemical Reviews·Osman DolucaVyacheslav V Filichev
Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michael A FischbachJon Clardy
Mar 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·C Richard Hutchinson
Aug 5, 2008·Nucleic Acids Research·Maria DucaPaola B Arimondo
Dec 18, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexandre S BoutorineAlya G Venyaminova
Mar 5, 2003·Chembiochem : a European Journal of Chemical Biology·O LentzenA Kirsch-De Mesmaeker
Jan 19, 2006·Chemistry : a European Journal·Rémy Lartia, Ulysse Asseline
Mar 1, 2013·Artificial DNA, PNA & XNA·Shuji IkedaAkimitsu Okamoto
Oct 21, 2011·The Journal of Physical Chemistry. B·Johanna Andersson, Per Lincoln
Jun 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marie FlammeMarcel Hollenstein
Oct 19, 2006·Dalton Transactions : an International Journal of Inorganic Chemistry·Johannes G Vos, John M Kelly
May 24, 2005·Dalton Transactions : an International Journal of Inorganic Chemistry·Shuo ShiLiang N Ji
Sep 11, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Stéphane Le GacAndrée Kirsch-De Mesmaeker
Sep 6, 2011·Nucleosides, Nucleotides & Nucleic Acids·Larysa DubeyIgor Dubey
Jan 30, 2018·Organic & Biomolecular Chemistry·Takaya SatoSeiichi Nishizawa
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·Alexandre S Boutorine, Christophe Escudé
Jan 28, 2020·Nucleosides, Nucleotides & Nucleic Acids·Yuta ItoYoshiyuki Hari
Jun 20, 2003·Current Opinion in Nephrology and Hypertension·Basil Hanss, Leslie A Bruggeman

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