Formation of dopamine and noradrenaline in rat vas deferens: comparison with guinea-pig vas deferens.

British Journal of Pharmacology
M Boadle-Biber, R H Roth

Abstract

1 The formation of [14C]-3,4-dihydroxyphenylalanine (DOPA) from [14C]-tyrosine, in the presence of the amino acid decarboxylase inhibitor, brocresine (3-hydroxy-4-bromobenzyloxyamine dihydrogen phosphate), was greatly enhanced in rat vasa deferentia depolarized by a KCl-enriched Krebs-Henseleit solution (52 mM KCl) compared with tissues maintained in unmodified Krebs-Henseleit solution. 2 When the conversion of tyrosine was allowed to proceed as far as catecholamine (brocresine absent) no significant difference was observed between the accumulation of [14C]-catecholamines (CA) in depolarized rat vasa deferentia and the accumulation in control (non-depolarized) tissues. 3 Endogenous CA levels in the depolarized rat vasa deferentia fell to 67% of the controls after a 1 h incubation period and to 53% at the end of 2 hours. 4 Chromatographic separation on Amberlite CG-120 columns of the newly synthesized CA and catechol metabolites from the rat vas deferens revealed that a very high proportion was present as dopamine. The percentage distribution after 1 h incubation in control Krebs-Henseleit was: noradrenaline (NA): 30.6 +/- 5.2; dopamine 56.9 +/- 5.9; acid metabolites: 12.8 +/- 1.1; and in KCl-rich Krebs-Henseleit, NA: 32; dopami...Continue Reading

References

Jan 1, 1970·Annual Review of Pharmacology·N Weiner
Nov 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A Alousi, N Weiner
Dec 1, 1972·British Journal of Pharmacology·M C Boadle-Biber, R H Roth
Jun 1, 1966·Life Sciences·R H RothU S von Euler
Dec 1, 1970·British Journal of Pharmacology·M C Boadle-BiberR H Roth
Dec 1, 1965·British Journal of Pharmacology and Chemotherapy·C C Chang, J C Chang
Feb 1, 1961·Acta Physiologica Scandinavica·U von EULER, F LISHAJKO

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Citations

Mar 14, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·S R SniderS Fahn
Sep 15, 1977·European Journal of Pharmacology·A JurkiewiczA D Guedes
May 26, 1998·European Journal of Pharmacology·H Morishita, T Katsuragi
May 1, 1979·Clinical and Experimental Pharmacology & Physiology·F M Tayo
Apr 1, 1978·British Journal of Pharmacology·H Miranda
Oct 1, 1989·Journal of Autonomic Pharmacology·M J RandT Dowding
Feb 1, 1981·Journal of Neurochemistry·C Bell, J S Gillespie

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