Formation of ethyl acetate by Kluyveromyces marxianus on whey during aerobic batch cultivation at specific trace element limitation

Applied Microbiology and Biotechnology
Thanet UritChristian Löser

Abstract

Kluyveromyces marxianus is able to transform lactose into ethyl acetate as a bulk product which offers a chance for an economical reuse of whey-borne sugar. Ethyl acetate is highly volatile and allows its process-integrated recovery by stripping from the aerated bioreactor. Extensive formation of ethyl acetate by K. marxianus DSM 5422 required restriction of yeast growth by a lack of trace elements. Several aerobic batch processes were done in a 1-L stirred reactor using whey-borne culture medium supplemented with an individual trace element solution excluding Mn, Mo, Fe, Cu, or Zn for identifying the trace element(s) crucial for the observed ester synthesis. Only a lack of Fe, Cu, or Zn restricted yeast growth while exclusion of Mn and Mo did not exhibit any effect due to a higher amount of the latter in the used whey. Limitation of growth by Fe or Cu caused significant production of ethyl acetate while limitation by Zn resulted in formation of ethanol. A lack of Fe or Cu obviously makes the respiratory chain inefficient resulting in an increased mitochondrial NADH level followed by a reduced metabolic flux of acetyl-SCoA into the citrate cycle. Synthesis of ethyl acetate from acetyl-SCoA and ethanol by alcoholysis is thus int...Continue Reading

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Citations

Oct 10, 2013·Applied Microbiology and Biotechnology·Thanet UritChristian Löser
Jan 19, 2013·World Journal of Microbiology & Biotechnology·L Amaya-DelgadoA Gschaedler
May 3, 2014·Applied Microbiology and Biotechnology·Christian LöserThomas Bley
Feb 26, 2013·Applied Microbiology and Biotechnology·Gustavo Graciano FonsecaAndreas Karoly Gombert
Oct 24, 2012·Journal of Biotechnology·Christian LöserThomas Bley
Dec 10, 2014·Applied Microbiology and Biotechnology·Christian LöserThomas Bley
Nov 16, 2016·Applied Microbiology and Biotechnology·Leelaram SantharamSurianarayanan Mahadevan
Apr 27, 2017·Journal of Industrial Microbiology & Biotechnology·A PentjussA Vigants
May 16, 2018·Engineering in Life Sciences·Christian LöserThomas Bley
Jul 29, 2018·Applied and Environmental Microbiology·Aleksander J KruisRuud A Weusthuis
Nov 17, 2020·Frontiers in Microbiology·Constantinos PatiniosServé W M Kengen

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