Formation of exudate droplets by Metarhizium anisopliae and the presence of destruxins

Mycologia
S HutwimmerW Burgstaller

Abstract

Nutritional conditions causing droplet exudation by Metarhizium anisopliae var. anisopliae were studied. Exudation in droplets occurred only on media with more than one carbon source and was highly dependent on the ratio of a well metabolized sugar such as trehalose and a nonpreferred sugar, in particular arabinose. Exuded droplets contained destruxin A, B and E in concentrations similar to those on submerged culture on Czapek Dox medium with equivalent C:N ratios but was clearly less than previously reported on standard Czapek Dox or Sabouraud dextrose broth. Destruxins also were found in agar samples from directly below mycelium and from up to 2 cm from the colony edge. Exudates retrieved from different media were proven to have Pr1 protease-related enzyme activity. Additional HPLC analysis indicated that droplets from diverse media did not differ in their sugar and acid content. A hypothesis is presented regarding the trigger for guttation in Metarhizium during growth under these conditions.

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Citations

Mar 13, 2014·Mycotoxin Research·Manfred Gareis, Christoph Gottschalk
Jun 2, 2015·Scientific Reports·Yong-Mei XingShun-Xing Guo
May 26, 2016·Journal of Industrial Microbiology & Biotechnology·Vincent P SicaNicholas H Oberlies
May 21, 2018·Journal of Applied Microbiology·E CastagnoliJ Kurnitski
Sep 28, 2018·Journal of Industrial Microbiology & Biotechnology·Chiraz Soumia M AmrineNicholas H Oberlies
Jul 25, 2017·Journal of Chemical Ecology·Kathrin Schmidt, Dieter Spiteller
May 22, 2019·Natural Product Reports·Nicholas H OberliesHuzefa A Raja

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