Formation of Fatty Acid-degrading, anaerobic granules by defined species.

Applied and Environmental Microbiology
W WuJ G Zeikus

Abstract

An endospore-forming, butyrate-degrading bacterium (strain BH) was grown on butyrate in monoxenic coculture with a methanogen. The culture formed dense aggregates when Methanobacterium formicicum was the methanogenic partner, but the culture was turbid when Methanospirillum hungatei was the partner. In contrast, a propionate-degrading, lemon-shaped bacterium (strain PT) did not form aggregates with Methanobacterium formicicum unless an acetate-degrading Methanosaeta sp. was also included in the culture. Fatty acid-degrading methanogenic granules were formed in a laboratory-scale upflow reactor at 35(deg)C fed with a medium containing a mixture of acetate, propionate, and butyrate by using defined cultures of Methanobacterium formicicum T1N, Methanosaeta sp. strain M7, Methanosarcina mazei T18, propionate-degrading strain PT, and butyrate-degrading strain BH. The maximum substrate conversion rates of these granules for acetate, propionate, and butyrate were 43, 9, and 17 mmol/g (dry weight)/day, respectively. The average size of the granules was about 1 mm. Electron microscopic observation of the granules revealed that the cells of Methanobacterium formicicum, Methanosaeta sp., butyrate-degrading, and propionate-degrading bacter...Continue Reading

References

Jul 1, 1991·Applied and Environmental Microbiology·J T GrotenhuisA J Zehnder
Apr 1, 1981·Journal of Bacteriology·W Kenealy, J G Zeikus
Jun 1, 1991·Applied and Environmental Microbiology·F A VisserE Conway de Macario

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Citations

May 23, 2008·Biotechnology and Bioengineering·Zhen-Peng ZhangAy Su
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Aug 5, 2010·Bioprocess and Biosystems Engineering·F Ilter Turkdogan-AydinolHurrem Bayhan
May 15, 2021·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Miao ZhangYafeng Li
Feb 1, 1997·Applied and Environmental Microbiology·M C VeigaJ G Zeikus
Jun 1, 1997·Microbiology and Molecular Biology Reviews : MMBR·B Schink
Mar 16, 2004·Water Research·L W Hulshoff PolP N L Lens

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