Formation of Free and Protein-Bound Heterocyclic Amines in Roast Beef Patties Assessed by UPLC-MS/MS

Journal of Agricultural and Food Chemistry
Jing ChenMaomao Zeng

Abstract

The effect of different roasting temperatures on the amounts of 17 heterocyclic amines (HAs) from seven categories of both free and protein-bound states in roast beef patties was assessed using an ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. There were increased amounts and more types of HAs detected at higher roasting temperatures. Nine free HAs were detected at 250 °C, including PhIP (14.34 ± 0.36 ng/g), DMIP (1.02 ± 0.07 ng/g), 1,5,6-TMIP (1.70 ± 0.08 ng/g), MeIQ (0.36 ± 0.01 ng/g), IQx (0.37 ± 0.04 ng/g), MeIQx (9.94 ± 0.61 ng/g), 4,8-DiMeIQx (0.90 ± 0.05 ng/g), norharman (6.03 ± 0.30 ng/g), and harman (2.60 ± 0.09 ng/g). Also, 37.32 ng/g of total free HAs was generated. Twelve protein-bound HAs were detected in roast beef patties at 250 °C, including PhIP (1.70 ± 0.13 ng/g), DMIP (2.33 ± 0.25 ng/g), 1,5,6-TMIP (3.62 ± 0.49 ng/g), MeIQ (5.47 ± 0.18 ng/g), IQ[4,5-b] (0.70 ± 0.03 ng/g) MeIQx (4.03 ± 0.41 ng/g), 4,8-DiMeIQx (0.67 ± 0.09 ng/g), MeAαC (19.51 ± 1.12 ng/g), AαC (2.91 ± 0.45 ng/g), norharman (1304.96 ± 110.73 ng/g), harman (400.85 ± 25.29 ng/g), and Phe-P-1 (0.81 ± 0.06 ng/g). The highest amount of protein-bound HAs was 2913.31 ng/g at 175 °C. PhIP tended to exist in a free s...Continue Reading

References

Jun 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G A GrossS Heyland
Jan 1, 1995·Environmental and Molecular Mutagenesis·R VikseJ S Felton
Jul 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M G KnizeJ S Felton
Apr 14, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Z BaloghA M Booren
Aug 30, 2000·International Journal of Cancer. Journal International Du Cancer·C MagagnottiL Airoldi
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·F ToribioL Puignou
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H FrandsenJ Alexander
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J AlexanderG Becher
Apr 10, 2004·Cancer Science·Takashi SugimuraMinako Nagao
Jun 23, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C L ChepanoskeK H Dingley
Mar 22, 2005·Chemical Research in Toxicology·Ricky D HollandRobert J Turesky
Oct 28, 2008·Journal of Agricultural and Food Chemistry·Armindo MeloIsabel M P L V O Ferreira
Mar 7, 2009·Journal of Agricultural and Food Chemistry·Mariana CostaIsabel M P L V O Ferreira
Dec 5, 2009·Molecular Nutrition & Food Research·Hiroyuki KataokaKurie Mitani
Jan 19, 2011·Meat Science·Kanithaporn PuangsombatJ Scott Smith
Oct 11, 2012·Journal of Separation Science·Arkadiusz SzterkAdam Cybulski
Oct 15, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Arkadiusz Szterk
Nov 26, 2015·Food Chemistry·Aurea Juliana Bombo TrevisanDeborah Helena Markowicz Bastos

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Citations

Mar 20, 2019·Mini Reviews in Medicinal Chemistry·Kianoush Khosravi-DaraniM Baghdadi
Jun 23, 2020·Journal of Agricultural and Food Chemistry·Rosario ZamoraFrancisco J Hidalgo

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