Formation of functional alpha3beta4alpha5 human neuronal nicotinic receptors in Xenopus oocytes: a reporter mutation approach

British Journal of Pharmacology
Paul J Groot-KormelinkLucia G Sivilotti

Abstract

1. The alpha5 subunit participates to the formation of native neuronal nicotinic receptors, particularly in autonomic ganglia. Like the related beta3 subunit, alpha5 forms functional recombinant receptors if expressed together with a pair of typical alpha and beta subunits, but its effect on the properties of the resulting alphabetaalpha5 receptor depends on the alpha and beta subunits chosen and on the expression system. We used a reporter mutation approach to test whether alpha5, like beta3, is incorporated as a single copy in human alpha3beta4alpha5 receptors expressed in oocytes. 2. As previously reported, the main indication of the presence of alpha5 in alpha3beta4alpha5(wt) was an increase in apparent receptor desensitization (compared with alpha3beta4 receptors). If the alpha3beta4alpha5 receptor bore a 9'T mutation in the second transmembrane domain of either alpha3 or beta4, alpha5 incorporation produced a decrease in ACh sensitivity (by 4 fold for alpha3(LT)beta4alpha5 vs. alpha3(LT)beta4 and by 40 fold for alpha3beta4(LT)alpha5 vs. alpha3beta4(LT)). The much greater effect observed in alpha3beta4(LT)alpha5 receptors accords with the hypothesis that alpha5 takes the place of a beta subunit in the receptor. 3. Introduc...Continue Reading

References

Jul 26, 1996·The Journal of Biological Chemistry·F WangJ Lindstrom
Nov 19, 1997·Progress in Neurobiology·C GottiF Clementi
Jun 23, 1998·The Journal of Biological Chemistry·P J Groot-KormelinkL G Sivilotti
Apr 14, 1999·The Journal of Physiology·M E Nelson, J Lindstrom
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·P J CorringerJ P Changeux
Feb 24, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·N Unwin

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Citations

Nov 19, 2002·Journal of Neurobiology·Mariella De Biasi
Jun 28, 2005·Trends in Neurosciences·Rashid GiniatullinJerrel L Yakel
May 19, 2004·The Journal of General Physiology·Paul J Groot-KormelinkLucia G Sivilotti
Jul 18, 2002·The Journal of Physiology·Ningshan WangAmos D Korczyn
Aug 29, 2012·Pharmacology & Therapeutics·Raymond HurstDaniel Bertrand
Sep 9, 2008·Trends in Neurosciences·Nelson P Barrera, J Michael Edwardson
Sep 25, 2003·British Journal of Pharmacology·Valeria CostaPaolo Carloni
Aug 14, 2008·The Journal of Comparative Neurology·Kathryn T O'LearyFrances M Leslie
Aug 21, 2007·Proteins·Marcelo O Ortells, Georgina E Barrantes
Sep 19, 2006·Biochemical Pharmacology·Ron C Hogg, Daniel Bertrand
Jun 20, 2003·The European Journal of Neuroscience·Silvia Di AngelantonioAndrea Nistri
Aug 15, 2014·BioMed Research International·Marion MeixnerVeronika Grau
Sep 27, 2008·Brain Research·Sandra RafalzikRüdiger Gerstberger
Feb 9, 2012·The Journal of Biological Chemistry·Bhagirathi Dash, Ronald J Lukas
Apr 16, 2003·Molecular Pharmacology·Ramiro SalasMariella De Biasi
Apr 26, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Miriam SciaccalugaSergio Fucile
Nov 20, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cristina DeflorioUwe Maskos
Apr 27, 2020·Cellular and Molecular Neurobiology·Kamilla MussinaOlena Filchakova
Jul 1, 2017·Journal of Mammary Gland Biology and Neoplasia·Nomundelger GankhuyagJe-Yoel Cho
Dec 22, 2020·Frontiers in Synaptic Neuroscience·Petra Scholze, Sigismund Huck
Apr 10, 2020·Neuropharmacology·Anant GharpureRyan E Hibbs

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