Formation of hemoglobin and albumin adducts of benzene oxide in mouse, rat, and human blood

Chemical Research in Toxicology
A B LindstromS M Rappaport

Abstract

Little is known about the formation and disposition of benzene oxide (BO), the initial metabolite arising from oxidation of benzene by cytochrome P450. In this study, reactions of BO with hemoglobin (Hb) and albumin (Alb) were investigated in blood from B6C3F1 mice, F344 rats, and humans in vitro. The estimated half-lives of BO in blood were 6.6 min (mice), 7.9 min (rats), and 7.2 min (humans). The following second-order rate constants were estimated for reactions between BO and cysteinyl residues of Hb and Alb [in units of L (g of Hb- or Alb-h)-1]: mouse Hb = 1.16 x 10(-)4, rat Hb = 15.4 x 10(-)4, human Hb = 0.177 x 10(-)4, mouse Alb = 2.68 x 10(-)4, rat Alb = 4.96 x 10(-)4, and human Alb = 5.19 x 10(-)4. These rate constants were used with BO-adduct measurements to assess the systemic doses of BO arising from benzene in vivo in published animal and human studies. Among rats receiving a single gavage dose of 400 mg of benzene/kg of body weight, the BO dose of 2.62 x 10(3) nM BO-h, predicted from Alb adducts, was quite similar to the reported AUC0-infinity = 1.09 x 10(3) nM BO-h of BO in blood. Interestingly, assays of Hb adducts in the same rats predicted a much higher dose of 14.7 x 10(3) nM BO-h, suggesting possible in situ ...Continue Reading

References

Sep 1, 1979·Journal of Toxicology and Environmental Health·D SammettR Snyder
Jan 1, 1976·Mutation Research·S Osterman-GolkarI Hällström
Oct 1, 1987·Toxicology and Applied Pharmacology·D A EastmondR D Irons
Jan 1, 1981·Mutation Research·L Ehrenberg, S Hussain
Jan 1, 1994·Critical Reviews in Toxicology·R Snyder, G F Kalf
Jan 1, 1996·Toxicology and Applied Pharmacology·E M KenyonM A Medinsky
May 15, 1996·Analytical Biochemistry·K Yeowell-O'ConnellS M Rappaport

❮ Previous
Next ❯

Citations

Nov 25, 1998·Chemico-biological Interactions·S WaidyanathaS M Rappaport
Feb 13, 2001·Toxicology·B K ParkM Pirmohamed
Oct 8, 1999·Toxicology Letters·S M Rappaport, K Yeowell-O'Connell
Jan 5, 2011·Molecular & Cellular Proteomics : MCP·He LiStephen M Rappaport
Feb 18, 2006·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Y S LinS M Rappaport
Dec 29, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Reza Hosseinzadeh, Ali Akbar Moosavi-Movahedi
Apr 20, 2011·Toxicology Letters·Stephen M RappaportEvan R Williams
Nov 28, 2009·Chemico-biological Interactions·David Ross, Hongfei Zhou
Jan 13, 2010·Chemico-biological Interactions·Bernard T GoldingMajid M Sadeghi
Jan 27, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M L GargasR G Tardiff
Feb 23, 2008·Chemico-biological Interactions·Suramya Waidyanatha, Stephen M Rappaport
Jan 1, 2000·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·R HanwayP B Farmer
Dec 19, 2016·Chemical Research in Toxicology·Gabriele Sabbioni, Robert J Turesky
Jun 20, 2002·Critical Reviews in Toxicology·Robert Snyder
Jan 26, 2013·Critical Reviews in Toxicology·Scott M ArnoldA Robert Schnatter
Oct 21, 2016·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·M Ariel Geer WallaceJoachim D Pleil
Jul 15, 2005·World Journal of Gastroenterology : WJG·Ming-Ling ChangJeng-Chang Chen
Dec 13, 2005·Carcinogenesis·Sungkyoon KimStephen M Rappaport
Nov 14, 2021·Archives of Toxicology·Gabriele Sabbioni, Billy W Day

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.