PMID: 7016859Jun 10, 1981Paper

Formation of hydroxypyruvaldehyde phosphate in human erythrocytes.

The Journal of Biological Chemistry
M Cogoli-Greuter, P Christen

Abstract

Human erythrocytes when incubated with [U-14C]glucose formed radioactively labeled hydroxypyruvaldehyde-P (CHOCOCH2OPO3(2-)). Using a "diagonal" chromatographic procedure on an anion exchange column for isolation and quantitative detection, up to 0.3 mumol of hydroxypyruvaldehyde-P/liter of packed cells was found. If glycolysis was blocked by inhibition of glyceraldehyde-3-P dehydrogenase with N-ethylmaleimide and iodoacetate, 0.7 mumol of hydroxypyruvaldehyde-P/liter of packed erythrocytes was detected. Hydroxypyruvaldehyde-P, found for the first time in eukaryotic cells, might be one of the long sought natural substrates of the glyoxalases.

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