PMID: 9188622Jul 1, 1997Paper

Formation of intracellular particles by hepatitis B virus large surface protein

Journal of Virology
Z XuT S Yen

Abstract

Hepatitis B virus small surface protein is synthesized as a transmembrane protein of the rough endoplasmic reticulum (RER) and then buds into the lumen in the form of subviral particles that are secreted. The closely related large surface protein is also targeted to the RER but is retained in a pre-Golgi compartment and cannot be secreted. It has been assumed that the large surface protein remains as a transmembrane RER protein and hence cannot form particles, possibly because of binding to a host factor on the cytosolic face of the RER membranes. We have reexamined this question and found the following results. (i) The retained large surface protein is associated not with RER but, rather, with a more distal compartment. (ii) Electron microscopy reveals intravesicular 20-nm particles, similar to those formed by the small surface protein. (iii) The large surface protein colocalizes with and binds to calnexin, an ER chaperone protein. Therefore, our results indicate that the large surface protein is capable of budding and forming particles, and hence its intracellular retention cannot be attributed to a cytosolic factor. We interpret the data as evidence that the large surface protein is retained by virtue of interacting with cal...Continue Reading

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Citations

Mar 23, 2007·Journal of Virology·Cheng HuangMing-Fu Chang
Sep 12, 2012·Medical Microbiology and Immunology·Reinhild Prange
Oct 2, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P Roingeard, C Sureau
May 1, 1999·Biological Chemistry·R PrangeH Löffler-Mary
Apr 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Philippe Roingeard
Nov 21, 2007·Journal of Biomedical Science·Willisa LiouSzecheng J Lo
Sep 13, 2012·Journal of Viral Hepatitis·V WilliamsE Gordien
Jul 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Petra RiedlReinhold Schirmbeck
Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C T BockC Trautwein
Feb 3, 2007·Journal of Virology·Romuald PatientPhilippe Roingeard
Jun 10, 2020·Cells·Mihaela-Olivia DobricaNorica Branza-Nichita
Dec 14, 2002·Journal of Hepatology·Ahmad S AbdulkarimMark A McNiven
Nov 30, 2004·Virus Research·Volker Bruss
Feb 9, 2012·Journal of Biomedical Science·Chih-Ming LinBing-Fang Chen

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