PMID: 9641268Jun 26, 1998Paper

Formation of keto and hydroxy compounds of linoleic acid in submitochondrial particles of bovine heart

Free Radical Biology & Medicine
H IwaseM Kobayashi

Abstract

To observe lipid peroxidation of additive-free submitochondrial particles, we incubated submitochondrial particles in the absence of exogenous irons and t-butyl hydroperoxide. After the incubation, the phospholipids were hydrolyzed by phopholipase A2, and the fatty acid constituents were analyzed by high-performance liquid chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry. Contrary to a commonly accepted theory, lipid peroxidation in the submitochondrial particles did not need the addition of NADH. In the phospholipid constituent fatty acids of the oxidized submitochondrial particles, derivatives of hydroperoxides of linoleic acid such as keto, hydroxy, trihydroxy, and hydroxyepoxy compounds were generated. Lipid peroxidation in the submitochondrial particles was not inhibited by the addition of catalase, superoxide dismutase, hydroxyl radical scavengers, or ethylenediaminetetraacetic acid, but was inhibited by the addition of KCN, antimycin-A, NADH, ubiquinol, deferoxamine mesylate, ascorbic acid, and alpha-tocopherol. The cardiolipin-cytochrome c lipid peroxidation system could mimic the lipid peroxidation of the submitochondrial particles, in terms of linoleic acid products and...Continue Reading

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Citations

Dec 4, 2003·Free Radical Biology & Medicine·Jos F H M Brouwers, Barend M Gadella
May 20, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Junko AdachiYasuhiro Ueno
May 9, 2000·Progress in Lipid Research·M SchlameM L Greenberg
Dec 5, 1998·Free Radical Biology & Medicine·H IwaseM Kobayashi
Nov 19, 2013·Biochemistry. Biokhimii︠a︡·Yu A VladimirovA V Alekseev
Mar 24, 2009·Journal of Molecular and Cellular Cardiology·Edward J LesnefskyCharles L Hoppel
Jun 3, 2008·Biochimica Et Biophysica Acta·Edward J Lesnefsky, Charles L Hoppel
Oct 26, 1999·Biochemical and Biophysical Research Communications·Y ShidojiK Yagi
Jun 2, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ana ReisP Domingues
Aug 2, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Junko AdachiYasuhiro Ueno
Mar 12, 2009·Journal of Cardiovascular Pharmacology·Genevieve C Sparagna, Edward J Lesnefsky

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