Formation of Large Scaffold-Free 3-D Aggregates in a Cell Culture Dish by Ultrasound Standing Wave Trapping

Ultrasound in Medicine & Biology
Misa NakaoKenjiro Takemura

Abstract

Cellular aggregates that mimic cell-cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated an aggregate formation system in which a 60-mm dish was set above a Langevin transducer via water. The transducer was excited at 110.8 kHz, and then C2C12 myoblasts were injected into the dish and trapped at the node position of the standing wave. The diameter and thickness of the formed aggregate were 8 and 2.7 mm, respectively, which are larger than those of aggregates formed previously by USWT. Moreover, we confirmed that >94% of cells constituting the aggregates survived 9 h, and the protein expression of cells was not altered significantly. This method can be applied to form aggregates with high functionality, which contributes to the development of biological research methodology.

Citations

Sep 25, 2019·Research : a Science Partner Journal·Hui ZhouHairong Zheng
Jan 7, 2021·International Journal of Molecular Sciences·Chikahiro Imashiro, Tatsuya Shimizu
Feb 20, 2021·Ultrasonics Sonochemistry·Genichiro FujiiKenjiro Takemura
Jun 11, 2021·Biotechnology and Bioengineering·Chikahiro ImashiroKenjiro Takemura
Jul 10, 2021·The Journal of the Acoustical Society of America·Taigo OyamaKenjiro Takemura

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