PMID: 9434855Jan 22, 1998Paper

Formation of micronuclei in incubated hen's eggs as a measure of genotoxicity

Mutation Research
Th Wolf, N P Luepke

Abstract

The formation of micronuclei (MN) is a widely used and accepted endpoint of genotoxicity testing. The micronucleus assay provides a simple and rapid indirect measure of the induction of structural or numerical chromosome aberrations. In this work we describe hen's eggs, incubated for 11 days, as ex vivo assay system for the detection of micronucleus formation in young erythrocytes (Hen's Egg Test for Micronucleus Induction, HET-MN). At this stage of development the chick embryo presents a high metabolic competency which allows an adequate activation of several types of promutagens, as previously reported by several authors. As all stages of maturing erythrocytes are present in the bloodstream of the chick embryo, we could conveniently use samples of peripheral blood for scoring micronuclei as well as for determining the ratio between mature and immature erythrocytes as a measure of an undisturbed erythropoiesis. The obtained blood smears were stained by a modified May-Gruenwald-Giemsa procedure and scored microscopically. The examinations were facilitated by using a semiautomatic image analysis system. We could demonstrate a strong increase of the micronucleus frequency after the administration of the promutagens diethylnitrosa...Continue Reading

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Citations

Mar 5, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Th WolfN-P Luepke
Jan 30, 2002·Mutation Research·Thorsten WolfNiels Peter Luepke
Jun 29, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gary M WilliamsUlrich Deschl
Nov 4, 2016·Toxins·Verônica da Silva CardosoMaria das Graças Miranda Danelli
Oct 30, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ercan DurmusMehmet Faruk Aydin
Aug 14, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tetyana KobetsGary M Williams
Jul 10, 2019·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·B Çelik, H Özparlak
Jun 4, 2021·Mutagenesis·Kerstin ReisingerRalph Pirow
Jun 7, 2008·Chemosphere·Tanise KnakieviczHenrique Bunselmeyer Ferreira

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