Formation of molecular complexes between a structurally defined M protein and acylated or deacylated lipoteichoic acid of Streptococcus pyogenes.

Journal of Bacteriology
I OfekE H Beachey

Abstract

The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell wall. We have investigated the possibility that LTA may become anchored to the cell surface by binding through its polyanionic backbone to positively charged regions of cell wall proteins. LTA was found to prevent the precipitation of partially purified HCl extracts of several strains of streptococci as well as a structurally defined streptococcal M protein molecule (pep M24) in 83% solutions of ethanol. The formation of complexes between LTA and M protein was demonstrated further by immunoelectrophoresis of pep M24 protein with increasing concentrations of radiolabeled LTA and by using antiserum against pep M24 to develop precipitin arcs. Pep M24 electrophoresed alone produced a single precipitin arc close to the origin. In contrast, when electrophoresed as a mixture with LTA or deacylated LTA, the M protein produced a second precipitin arc toward the anode coinciding with the area of migration of the radioactive L...Continue Reading

References

Mar 28, 1975·Science·A J Wicken, K W Knox
Jun 1, 1977·Infection and Immunity·R H Johnson, K L Vosti
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E H BeacheyA H Kang
May 1, 1975·The Journal of Experimental Medicine·I OfekG L Campbell
Oct 1, 1975·Journal of Bacteriology·R J DoyleF E Young
Feb 1, 1979·European Journal of Biochemistry·J Gmeiner, S Schlecht
Jan 1, 1978·Advances in Experimental Medicine and Biology·G D ShockmanM Mychajlonka
Apr 1, 1976·The Journal of Experimental Medicine·E H Beachey, I Ofek
Mar 1, 1974·Bacteriological Reviews·E N Fox
May 1, 1974·Journal of Bacteriology·T H ChiuD Platt
Jun 1, 1973·Bacteriological Reviews·K W Knox, A J Wicken
Nov 1, 1969·Analytical Biochemistry·R R Lauwerys
Feb 1, 1980·Infection and Immunity·I van de Rijn, R E Kessler
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G N PhillipsV A Fischetti
Jul 1, 1952·The Journal of Experimental Medicine·R C LANCEFIELD, G E PERLMANN

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Citations

Jan 1, 1988·Folia Microbiologica·L E RavdonikasA A Totolian
Feb 1, 1984·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·H MiörnerG Kronvall
Sep 12, 2014·BMC Pharmacology & Toxicology·Christian WunderlichManfred Kietzmann
Sep 1, 1991·Mycopathologia·S GottliebE Segal
Jan 1, 1990·Archives of Microbiology·G J Ruhland, F Fiedler
Dec 14, 2011·Journal of Bacteriology·Keiji Richard KimuraShigetada Kawabata
Jul 1, 1984·Scandinavian Journal of Immunology·L BjörckR Sundler
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Francis C Neuhaus, James Baddiley
Dec 9, 2000·FEMS Microbiology Letters·S SelaA Barzilai
Apr 28, 2009·The Journal of Biological Chemistry·Irina G SavaJohannes Huebner
Jun 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·D N KurlC Schalén
Sep 27, 2019·Current Drug Targets·Victoria A Ploplis, Francis J Castellino
Nov 7, 2000·FEMS Immunology and Medical Microbiology·P A LambertT S Elliott
Oct 26, 2018·Proceedings of the National Academy of Sciences of the United States of America·Takashi ImaiSho Yamasaki
Mar 22, 2001·Advanced Drug Delivery Reviews·U B Kompella, V H Lee
May 1, 1991·Infection and Immunity·M G CaparonJ R Scott
Jul 1, 1989·Clinical Microbiology Reviews·V A Fischetti
Feb 1, 1994·Infection and Immunity·J R Wang, M W Stinson
Nov 1, 1988·Antimicrobial Agents and Chemotherapy·D M Schifferli, E H Beachey
Apr 1, 1983·Infection and Immunity·M M Carruthers, W J Kabat
Apr 1, 1983·Infection and Immunity·P LancyS C Holt
Jan 1, 1988·Clinical Microbiology Reviews·H D Isenberg
Jan 1, 1985·Infection and Immunity·H S CourtneyE H Beachey
Feb 1, 1986·Infection and Immunity·S L JosephsonR E Cohen
Jan 1, 1985·Infection and Immunity·H J Op den CampJ H Veerkamp
Jun 1, 1992·Infection and Immunity·D L HastyR J Doyle
Nov 1, 1991·Journal of Bacteriology·I C Sutcliffe, N Shaw
Mar 1, 1983·Infection and Immunity·E RosenbergM Rosenberg
Jul 1, 1988·Infection and Immunity·E J Bergey, M W Stinson

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