Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization.

Cytotechnology
Hiroyuki TanakaYukihiro Shoyama

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.

References

Sep 1, 1988·Chemical & Pharmaceutical Bulletin·H YamaguchiT Fuwa
Dec 1, 1993·Journal of Natural Products·H TanakaY Shoyama
Jul 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·B F ERLANGER, S M BEISER
Jan 1, 1995·Cytotechnology·Y ShoyamaH Murakami

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Citations

May 25, 2004·The American Journal of Chinese Medicine·P LoungratanaY Shoyama
Jan 8, 2004·Biological & Pharmaceutical Bulletin·Shu-Hang ZhuYukihiro Shoyama
Apr 2, 2009·Biological & Pharmaceutical Bulletin·Eun Ji JooYeong Shik Kim
Oct 6, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yukihiro Shoyama
Apr 21, 2012·International Journal of Analytical Chemistry·Hiroyuki TanakaYukihiro Shoyama
Oct 3, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takuhiro Uto
Feb 4, 2006·Phytochemical Analysis : PCA·Hiroyuki TanakaYukihiro Shoyama
Nov 23, 2017·Journal of Natural Medicines·Seiichi SakamotoSatoshi Morimoto
Feb 3, 2006·Journal of Chromatography. a·W Oleszek, Z Bialy

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