PMID: 5257000Mar 1, 1969Paper

Formation of neuromuscular junctions in embryonic cell cultures

Proceedings of the National Academy of Sciences of the United States of America
Y ShimadaA A Moscona

Abstract

The formation of nerve-muscle junctions in monolayer cultures of embryonic muscle and spinal cord cells is described. Muscle-forming cells (myoblasts) from leg muscles of 12-day chick embryos were separated with trypsin and cultured on a collagen substrate for two days. Suspensions of ventral spinal cord cells from six-day chick embryos were then plated over the differentiating muscle cells. The cultures were subsequently examined by electron microscopy and in silver-stained light microscopic preparations. After 10-12 days in culture, irregular thickenings were observed along the nerve cell processes in contact with the muscle fibers which by that time had undergone advanced differentiation (myogenesis). By electron microscopy it was demonstrated that many of the nerve-muscle contacts had the characteristics of a synapse. Some aspects of the fine structure of these junctions are described. The possibilities raised by these findings with respect to innervation mechanisms and specificities in nerve-muscle interactions are briefly discussed.

References

Nov 1, 1967·The Journal of Cell Biology·Y ShimadaA A Moscona
Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·S D Hauschka, I R Konigsberg
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Yaffe
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Citations

Jan 1, 1970·Zeitschrift für Zellforschung und mikroskopische Anatomie·E R Peterson, S M Crain
Mar 1, 1975·Developmental Biology·Y Shimada, D A Fischman
Oct 1, 1990·Brain Research. Developmental Brain Research·C S CohanM H Xia
Jan 1, 1976·Progress in Neurobiology·H L Atwood
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·N W Seeds, A E Vatter
Mar 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R L SeecofJ Donady
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·C K GovindF Lang
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S MasukoY Shimada
Feb 1, 1972·The Journal of Cell Biology·A I Farbman
Jan 1, 1973·The Journal of Cell Biology·R R Minor
Jun 1, 1973·Journal of Neurology, Neurosurgery, and Psychiatry·J H Peacock, P G Nelson
Jan 1, 1990·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M MüntenerH Walt
Oct 15, 1979·Experimental Cell Research·C R WalkerR C Strohman
Oct 1, 1973·Journal of Cellular Physiology·S N Lawson, T J Biscoe
Jun 1, 1972·Journal of Cellular Physiology·M KanoK Ishikawa
Mar 22, 1974·Annals of the New York Academy of Sciences·D FambroughA K Ritchie
Oct 12, 2020·Annals of the New York Academy of Sciences·María José Castellanos-MontielItzel Escobedo-Avila
Jan 1, 2021·Journal of Neurochemistry·Rianne de JonghR Jeroen Pasterkamp
Aug 1, 1974·Differentiation; Research in Biological Diversity·A L Harvey, W F Dryden

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