Formation of nuclear bodies of Arabidopsis CRY2 in response to blue light is associated with its blue light-dependent degradation.

The Plant Cell
Xuhong YuChentao Lin

Abstract

Arabidopsis thaliana cryptochrome 2 (CRY2) mediates photoperiodic promotion of floral initiation and blue light inhibition of hypocotyl elongation. It has been hypothesized that photoexcitation derepresses CRY2 by disengaging its C-terminal domain from the N-terminal PHR domain. To test this hypothesis, we analyzed activities of CRY2 fused to green fluorescent protein (GFP) at either the N terminus (GFP-CRY2) or the C terminus (CRY2-GFP). While GFP-CRY2 exerts light-dependent biochemical and physiological activities similar to those of the endogenous CRY2, CRY2-GFP showed constitutive biochemical and physiological activities. CRY2-GFP is constitutively phosphorylated, it promotes deetiolation in both dark and light, and it activates floral initiation in both long-day and short-day photoperiods. These results are consistent with the hypothesis that photoexcited CRY2 disengages its C-terminal domain from the PHR domain to become active. Surprisingly, we found that CRY2-GFP, but not GFP-CRY2, formed distinct nuclear bodies in response to blue light. Compared with GFP-CRY2 or the endogenous CRY2, CRY2-GFP degradation was significantly retarded in response to blue light, suggesting that the nuclear bodies may result from accumulatio...Continue Reading

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Citations

Dec 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xu LiChentao Lin
Oct 12, 2010·Plant Physiology·Martijn van ZantenPaul Fransz
Sep 29, 2011·Plant Physiology·Elise K Van BuskirkMeng Chen
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Dec 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shangwei ZhongHongwei Guo
Jan 27, 2016·Journal of Plant Research·Bobin LiuYoshito Oka
Oct 11, 2011·Trends in Plant Science·Hongtao LiuChentao Lin
Jul 17, 2015·Plant Signaling & Behavior·Nathalie JourdanMargaret Ahmad
Feb 13, 2013·The Plant Journal : for Cell and Molecular Biology·Vera HerbelAlfred Batschauer
Apr 12, 2013·The Plant Journal : for Cell and Molecular Biology·Andrea SchraderJoachim F Uhrig
Jun 15, 2016·Plant Science : an International Journal of Experimental Plant Biology·Mingmin JiangHuoying Chen
Nov 5, 2014·Journal of Pineal Research·Jerry Vriend, Russel J Reiter
Jul 3, 2015·Frontiers in Molecular Biosciences·Thea Ziegler, Andreas Möglich
Apr 25, 2012·Molecular Plant·Martijn van ZantenPaul Fransz
Dec 18, 2014·Plant & Cell Physiology·John M ChristieStuart Sullivan
Oct 25, 2013·Preparative Biochemistry & Biotechnology·Canan OzyurtAzmi Telefoncu
Jul 26, 2016·Frontiers in Plant Science·Liang YangZecheng Zuo
Nov 14, 2015·Journal of Integrative Plant Biology·Wenming ZhaoBo Ren
Nov 16, 2016·Science·Qin WangChentao Lin
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Oct 19, 2010·Biologie aujourd'hui·Federico Tessadori
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Aug 2, 2019·Plant, Cell & Environment·James Ronald, Seth J Davis
Nov 20, 2016·Photochemistry and Photobiology·Zhaohe YangYoshito Oka
Mar 15, 2020·Annual Review of Plant Biology·Qin Wang, Chentao Lin

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