Formation of osteoclast-like cells from peripheral blood of periodontitis patients occurs without supplementation of macrophage colony-stimulating factor

Journal of Clinical Periodontology
Stanley T S TjoaVincent Everts

Abstract

To determine whether peripheral blood mononuclear cells (PBMCs) from chronic periodontitis patients differ from PBMCs from matched control patients in their capacity to form osteoclast-like cells. PBMCs from 10 subjects with severe chronic periodontitis and their matched controls were cultured on plastic or on bone slices without or with macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor-kappaB ligand (RANKL). The number of tartrate-resistant acid phosphatase-positive (TRACP(+)) multinucleated cells (MNCs) and bone resorption were assessed. TRACP(+) MNCs were formed under all culture conditions, in patient and control cultures. In periodontitis patients, the formation of TRACP(+) MNC was similar for all three culture conditions; thus supplementation of the cytokines was not needed to induce MNC formation. In control cultures, however, M-CSF or M-CSF/RANKL resulted in higher numbers compared with cultures without cytokines. Upregulations of osteoclast marker mRNA cathepsin K and carbonic anhydrase II confirmed the osteoclastic character. Bone resorption was only observed when PBMCs were cultured in the presence of M-CSF and RANKL. Our data indicate that PBMCs from periodontitis patients do not ...Continue Reading

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Citations

Dec 2, 2009·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·P D'AmelioG C Isaia
Jun 14, 2013·Clinical & Developmental Immunology·Adriana Di BenedettoMaria Grano
Nov 20, 2018·Journal of Leukocyte Biology·Corneliu SimaMichael Glogauer
Mar 19, 2014·PloS One·Mercedes F RiveraLakshmyya Kesavalu
Apr 18, 2009·Molecular Cancer Research : MCR·Teun J de VriesJenneke Klein-Nulend
Aug 13, 2020·Molecular Genetics and Metabolism·P A RozenfeldM V Delpino

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