PMID: 8597585Feb 16, 1996Paper

Formation of pregnenolone- and dehydroepiandrosterone-fatty acid esters by lecithin-cholesterol acyltransferase in human plasma high density lipoproteins

Biochimica Et Biophysica Acta
B LavalléeA Bélanger

Abstract

Pregnenolone- (PREG-), and dehydroepiandrosterone- (DHEA-) fatty acid esters (FA) are present in human plasma, where they are associated with lipoproteins. Because plasma has the ability to form PREG-FA and DHEA-FA in vitro from their unconjugated steroid counterparts, we postulated that the LCAT enzyme might be responsible for their formation. Here we show that lecithin-cholesterol acyltransferase (LCAT) has PREG and DHEA esterifying activities. First, VLDL, IDL, LDL, and HDL were isolated by the sequential ultracentrifugation micromethod from the plasma of fasting men and women and tested for their ability to form PREG-FA, DHEA-FA, and cholesteryl esters in vitro from their respective unconjugated counterparts. The results showed that the three steroids were esterified only in HDL subfractions. The rate of tritiated PREG esterification was clearly higher than that of tritiated cholesterol and DHEA, both in total plasma and isolated HDL, and no gender difference was observed. Second, human and guinea pig LCAT were purified and used in phosphatidylcholine-reconstituted vesicles containing human apoAI to show their ability to esterify tritiated cholesterol, PREG, and DHEA in the absence of unlabeled steroid. The amount of choles...Continue Reading

References

Sep 30, 1991·Biochemical and Biophysical Research Communications·R Roy, A Bélanger
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Jan 1, 1989·Journal of Steroid Biochemistry·R Roy, A Bélanger
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May 19, 1995·The Journal of Biological Chemistry·S E SzedlacsekT Nishida

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Citations

Oct 8, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Dobiásová, J J Frohlich
Jul 1, 1997·Baillière's Clinical Endocrinology and Metabolism·F Svec
Jun 24, 2011·Chemical Reviews·Jonathan Z Long, Benjamin F Cravatt
Apr 5, 2012·The Journal of Biological Chemistry·Maximillian A RogersTa-Yuan Chang
Sep 15, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Maximillian A RogersTa-Yuan Chang
May 1, 1999·Biochemical and Biophysical Research Communications·X ColletC J Fielding
Feb 15, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Feng WangMatti J Tikkanen
Jun 29, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Veera VihmaHerman Adlercreutz
Jun 17, 1998·Endocrine Reviews·R B Hochberg
Jul 31, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Lina SchifferKarl-Heinz Storbeck
Apr 8, 1999·Progress in Lipid Research·A Jonas
Jan 5, 2002·Journal of Chromatography. a·A MarwahH Lardy

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